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RNF41 (NM_005785) Human Untagged Clone

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SKU
SC111140
RNF41 (untagged)-Human ring finger protein 41 (RNF41), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RNF41
Synonyms FLRF; NRDP1; SBBI03
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111140 sequence for NM_005785 edited (data generated by NextGen Sequencing)
ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA

Clone variation with respect to NM_005785.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005785 unedited
NGGTCAAAATTTGTATACGACTCCTATAGGCGGCCGCGAATTCGCCGGGGCGGGGCAAAG
GATCCGTCTGAGGCGGCGACGAGTGAGTACTCCTGATTGTGACATCACATTCATCCCCTG
GGCGATGGAGCTTGTCACTGGGAAGGAATACTCAGTCGGAGAATAGCCAACAAGATGGGT
TACTGGGAGAATCTCTTCAGTGGCACTGAGTGGAGGCATCAGGGGGTTGGAGCCTTGTGA
ACAGGGAACCTGCCCCCCAACACTTGGAAGGACCTGGGTTTCAGTGATGAGACATGGGGT
ATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATTTGCAGTG
GAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAACGCCTGCA
TCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTGACGGTCG
CCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAGATTGCCT
GTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATGTCTCACC
TCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGTGGCCTGG
AGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCAGTGGTAC
AGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACANACACCAGC
TGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCNATCCGCAGTG
TCAACCCCACCTTCAGACCCTGGNAGAGACATTGATACCACGAGATCCTANAGTGGGTGA
ACTCCCTTCANCANNCAGATGACCGCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005785 unedited
TGTACGCGGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTTTTAGAGATTGAGTAAA
AGGTTTATTATTAGTGCAGTCAACACCATGGAACAGCACATACAACACAACCAGCAACCT
GCAGAGACACTAGTGCAAAGGGTAGGGAAGCCTTTCACTGAGCTTCCTGGCTCCATCTGA
GGGTAAGGACAGGACAGTATGAGCCTTGGTTAAGGCAGGTAGGGGAAAGGGGAGTGGAAG
AAATGTAGTAACCAGAGTAAGTATAGCAGCGTTTTCAAATTCCTGAGCACAATGTCCCAG
AGCTGGAACCCTACTCCCCTCAAGCTTTCCACCCCAATCCCAGTGGAGCCATGATCCAAC
TACCCAGACCTGCAGCAAGCTAGCCTGGAATAAAATTCTGAGAGGAAGCCATTACATGGT
GGGGAGGAGCCTTTCTATCTCCAACCACACTCCCACCTCCATATTAAAATAATCACACCT
ATTATCTCTCCCCTTCTCTTACTCTGCCAGTCACTCTAGGGATCAAGGTATACCCCAGGT
AACCTGAAATGGAACTGAAGTCCCCAGGGGGGTGTGGGTGAGACACGGCACAGCCTGTAC
TTAGAGATAAAATTTCTGATTTGCATGGNGAAGCTTAGAACCCAGCTATACAGCTNCAAG
GAAACTTGGGGAATGGGGGAGTTGCCTTCAAAATGAATACTCTAAATCCAGAGTTTTATG
ATAAAGCCCTGGAAAGGCTAGGAGGAAATTTTTCCAGAGGGCACAAGCTTCGATGCTGGA
CTGTCATATCCTTCACAGAAGAGAGAAGCTCCTCATTCAGTTGGGTTACACGAGTTTGGG
CAAATTCGTAAGTTAGCCGAGACTCCTTGGCTTTTCATATTAAGCCAAGGTGGCTGAAAG
GAAAATTAAAACACCCCATCCTGTCAACCTTCCAACCCCCATAGGAACTAAAAAAAGTCA
GTGGATTTTTTCTTTT
Restriction Sites NotI-NotI |
ACCN NM_005785
Insert Size 3220 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005785.2, NP_005776.1
RefSeq Size 3286 bp
RefSeq ORF 954 bp
Locus ID 10193
UniProt ID Q9H4P4
Cytogenetics 12q13.3
Domains RING
Protein Pathways Endocytosis
Summary This gene encodes an E3 ubiquitin ligase. The encoded protein plays a role in type 1 cytokine receptor signaling by controlling the balance between JAK2-associated cytokine receptor degradation and ectodomain shedding. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2011
Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_005785" in other vectors (6)
SKU Description Size Price
RC217918 RNF41 (Myc-DDK-tagged)-Human ring finger protein 41 (RNF41), transcript variant 1 10 ug
$450.00
RC217918L1 Lenti ORF clone of Human ring finger protein 41 (RNF41), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC217918L2 Lenti ORF clone of Human ring finger protein 41 (RNF41), transcript variant 1, mGFP tagged 10 ug
$750.00
RC217918L3 Lenti ORF clone of Human ring finger protein 41 (RNF41), transcript variant 1, Myc-DDK-tagged 10 ug
$750.00
RC217918L4 Lenti ORF clone of Human ring finger protein 41 (RNF41), transcript variant 1, mGFP tagged 10 ug
$750.00
RG217918 RNF41 (tGFP-tagged) - Human ring finger protein 41 (RNF41), transcript variant 1 10 ug
$650.00

file-alt Citations

Decreased RNF41 expression leads to insulin resistance in skeletal muscle of obese women
Breuker, Amouzou, Fabre et al
Metab. Clin. Exp. (2018)
AI Summary: OriGene's untagged RNF41 cDNA clone (NM_005785) in the PCMV6-XL4 vector and the empty PCMV6-XL4 vector were used to overexpress RNF41 in primary human skeletal muscle cells, enabling the study to demonstrate that increased RNF41 expression improves insulin response and interferon pathway activation in insulin-resistant myotubes.
Snippet: For RNF41 overexpression, three days after induction of differentiation, OIR cells were transfected with 250 ng of untagged cDNA cloned (NM_ 005785) in PCMV6-XL4 vector (vRNF41) or with empty PCMV6-XL4 vector (eV) (OriGene, Rockville, USA) mixed with turbofectin
Showing 1-1 of 1 papers.
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