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MDFI (NM_005586) Human Untagged Clone

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SKU
SC110947
MDFI (untagged)-Human MyoD family inhibitor (MDFI)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MDFI
Synonyms I-MF; I-mfa
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC110947 sequence for NM_005586 edited (data generated by NextGen Sequencing)
ATGTACCAGGTGAGCGGCCAGCGCCCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGC
GCAGCCCCGGGCCCAGCCCAGACCCTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGA
TCCACTCACCCTGCGGAGGCAGCACCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCC
ATGCCCCAAGGCAATGGCCCTGGCATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTC
CCCACAGAAGCTGTGACATGCCAGCCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTG
CCGAATGACTCTGGCCACCCCTCAGAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCC
CTGGGTGGCCCCAAGGCCCACCGGAAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGC
AGCAAGAAGAGTAAGAGCAGCAGCAAATCCACCACCTCCCAGATCCCCCTCCAGGCACAG
GAAGACTGCTGTGTCCACTGCATCCTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGC
AACATCGTCCTGGACTGCGCCACCTGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGC
TGCTGCTGCTGTGGCTCTGGCGAGTGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGC
GGCATCCTGGATGCCTGCTGCGAGTCCGCGGACTGCCTGGAGATCTGCATGGAGTGCTGT
GGGCTCTGCTTCTCCTCCTGA

Clone variation with respect to NM_005586.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005586 unedited
GGGGCAACACTTGGTATTTGTNAAACGACTTACTANAGGGNNCGGCCGCGNAATTCGGCA
CGAGGGGAGAAAGCGCGAGTGAGAGGGGAAGGGGCGCCAGGCGAGCACCCGGGAGCCAGC
GGGACCTGGGCAGGGGCGCCCGGAGCAGGCGCGCATGGCGGGCCCCGCGCGGGGATCCGG
CTGGAAGAGAGCGTAGCACGGCTCGCACGAGTCCGGGGCCGATGTACCAGGTGAGCGGCC
AGCGCCCCTCTGGCTGCGACGCGCCCTATGGAGCCCCCAGCGCAGCCCCGGGCCCAGCCC
AGACCCTATCCCTCCTTCCTGGGCTGGAGGTAGTAACAGGATCCACTCACCCTGCGGAGG
CAGCACCAGAGGAGGGCTCCCTGGAGGAGGCGGCAACCCCCATGCCCCAAGGCAATGGCC
CTGGCATCCCCCAGGGCCTGGACAGCACTGACCTCGACGTCCCCACAGAAGCTGTGACAT
GCCAGCCTCAGGGGAACCCCTTGGGCTGCACCCCACTTCTGCCGAATGACTCTGGCCACC
CCTCAGAGCTGGGCGGCACCAGACGGGCGGGGAATGGTGCCCTGGGTGGCCCCAAGGCCC
ACCGGAAGTTGCAGACACACCCATCTCTCGCCAGCCAGGGCAGCAAGAAGAGTAAGAGCA
GCAGCAAATCCACCACCTCCCAGATCCCCCTCCAGGCACAGGAAGACTGCTGTGTCCACT
GCATCCTGTCCTGCCTGTTCTGCGAGTTCCTGACGCTGTGCAACATCGTCCTGGACTGCG
CCACCTGTGGCTCCTGCAGCTCGGAGGACTCGTGCCTCTGCTGCTGCTGCTGTGGCTCTG
GNCGAGTGTGCCGACTGCGACCTGCCCTGCGACCTGGACTGCGGCATNCTGGATGCCTGC
TGCGAGTCCGCGGACTGCTGGAGAT
Restriction Sites Please inquire |
ACCN NM_005586
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005586.2, NP_005577.1
RefSeq Size 1558 bp
RefSeq ORF 741 bp
Locus ID 4188
UniProt ID Q99750
Cytogenetics 6p21.1
Protein Families Transcription Factors
Summary This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. provided by RefSeq, Jul 2008
Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. It encodes the longer isoform (1). Variants 1, 3, and 4 encode the same isoform (1).
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Documents
Resources
"NM_005586" in other vectors (4)
SKU Description Size Price
RC203168 MDFI (Myc-DDK-tagged)-Human MyoD family inhibitor (MDFI) 10 ug
$289.00 $300.00
RC203168L3 Lenti ORF clone of Human MyoD family inhibitor (MDFI), Myc-DDK-tagged 10 ug
$600.00
RC203168L4 Lenti ORF clone of Human MyoD family inhibitor (MDFI), mGFP tagged 10 ug
$600.00
RG203168 MDFI (tGFP-tagged) - Human MyoD family inhibitor (MDFI) 10 ug
$489.00 $500.00

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