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TFIIB (GTF2B) (NM_001514) Human Untagged Clone

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SKU
SC110914
GTF2B (untagged)-Human general transcription factor IIB (GTF2B)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TFIIB
Synonyms TF2B; TFIIB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_001514, the custom clone sequence may differ by one or more nucleotides


ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAG
TGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGA
TGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTG
ACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGC
ATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTC
TCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTC
TAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
ACTGGGGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACAC
ATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGC
TATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTTCCTACAGACT
TCAAATTTGACACCCCAGTGGACAAACTACCACAGCTATAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_001514 unedited
CGTTTACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGTTGTGT
CTTGTTGCGGGCACCGCAGTCGCCGTGAAGATGGCGTCTACCAGCCGTTTGGATGCTCTT
CCAAGAGTCACATGTCCAAACCATCCAGATGCGATTTTAGTGGAGGACTACAGAGCCGGT
GATATGATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGATGTGGGATCT
GAATGGCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCT
CAGAATCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCT
GCAAGTTTTGACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCT
GATCGGGCAATGATGAATGCATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTA
CCTCGAAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTATATGAACAGAAGAGC
CTGAAGGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAA
GAAGGGGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTCTAAGAAAGAA
ATTGGTCGGTGGTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACA
ACTGGGGACTTCATGTCCAGGTTCTGGTCCAACCTTTGTCTTCCTNNAACAGTACAGATG
GCAGCTACACATATAGCCCCGTAAGCTGTGGAATTGGACTTGGTTCCTGGGNNAGAGCCC
CATCTCTGTGGCAGCGGCAGCTATTTACATGGCCTCACAGCCATCAGCTGGAAAGAGGAC
CCACAAGAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001514 unedited
TGTACCGCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTTTGAAAAA
TGTTTTATTCAAGATTACATAATTTCAAATATCTTCCAAAATACAGTTTCCTAGATTGCT
ACAAAAGAAATTTGATAAGAAATTTAAATCATTAAATATGTTTCACTAGTATATACATAC
AGAATGCCAAGCAATAGTATTCAGCCCTGGAATGCGTACCATGTCTTTTGTTTTTTCTCA
TGAAAGGCTCAACCCAGCATTTTGCATAGGCTATGTACAACAGGCAAAGTTTTGTATTCA
AGAATTTGACGTACGCTGCCTCAATTTATAGCCTGTGGCACTTCGCCCACTGGGGTGTCA
CATTTGAAGCCTGTAGGAACCAGATTTGGGGCTCGAGGATAGACCAGTCTATAGGACTGT
CTGATTGTACCATCAGCAACACCAGCATTCTCTCCAATTTCCTTCTGCGTCCTCTCTTAC
CTGATGCCCGCGAGGCCATGCCAATAGCTGTCGTTGCCACAGACACGGCCGCTCCTCCTT
ATGAGTCCCGTCCCCCTCACAGCCTTACGGGCTACATGTGTAGCTGCCATCCGCCCACGG
CTAGGAAGACAAAGCGTCGCCACCGAACCTGGACACGAATTCCCTCAGTTGTCATTCACA
CCCACATTGGTTCCTAGCGCCTTTCAACTCAGTTCCAACCACCGCCCCCCCCCTCTCTAT
CAACCCGGCCCCGGCACATATTTTTTTCACCGCCCTAAGACCCCCCCCCCTGCCTTAAGC
CATATACTATTCTTTTCCCTCCTTTTTCCCCACCACCCCCTCCCCTCCAGGCTTCTCTTG
GAACCAAACTTGTTTCGCACCCATATCCGTTCGCCAACCTCTCTCGCGACGGTTCATGCT
CTCCCCCGCCCGGCAGGTGACTATTTGAAGCACTTATCACTCGCCACAGCACCGCCCCGT
TTTCACACCGCCCTCTAACTGCCCTCCCGTAACCTGGGCCCCGCTTTTCCT
Restriction Sites NotI-NotI |
ACCN NM_001514
Insert Size 1350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001514.3, NP_001505.1
RefSeq Size 1304 bp
RefSeq ORF 951 bp
Locus ID 2959
UniProt ID Q00403
Cytogenetics 1p22.2
Domains CYCLIN, transcript_fac2
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Basal transcription factors
Summary This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001514" in other vectors (5)
SKU Description Size Price
RC204607 GTF2B (Myc-DDK-tagged)-Human general transcription factor IIB (GTF2B) 10 ug
$300.00
RC204607L3 Lenti ORF clone of Human general transcription factor IIB (GTF2B), Myc-DDK-tagged 10 ug
$600.00
RC204607L4 Lenti ORF clone of Human general transcription factor IIB (GTF2B), mGFP tagged 10 ug
$600.00
RG204607 GTF2B (tGFP-tagged) - Human general transcription factor IIB (GTF2B) 10 ug
$489.00 $500.00
SC321111 GTF2B (untagged)-Human general transcription factor IIB (GTF2B) 10 ug
$300.00

file-alt Citations

Acute Targeting of General Transcription Factor IIB Restricts Cardiac Hypertrophy via Selective Inhibition of Gene Transcription
Sayed, Yang, He et al
Circ Heart Fail (2014)
AI Summary: OriGene contributed human TFIIB cDNA (accession # NM_001514) and CDK9 cDNA (accession # NM_001261.2), which were used to generate adenoviral constructs for investigating the roles of these genes in cardiac hypertrophy.
Snippet: adenovirus under CMV and used as control for all adenoviral microRNA constructs. TFIIB accession # NM_001514 (Origene) and CDK9 accession # NM_001261.2 (Origene) human cDNA with or without the 3'UTR containing the miR-1 target site were cloned into adenovirus ... Cite SaveSaving
Showing 1-1 of 1 papers.
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