RBFOX1 (NM_018723) Human Untagged Clone

SKU SC110486

RBFOX1 (untagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 1 (RBFOX1), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: RBFOX1
• Synonyms: 2BP1; A2BP1; FOX-1; FOX1; HRNBP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110486 sequence for NM_018723 edited (data generated by NextGen Sequencing); ATGAATTGTGAAAGAGAGCAGCTAAGGGGTAATCAGGAAGCAGCCGCTGCCCCTGACACA; ATGGCTCAGCCTTACGCTTCGGCCCAGTTTGCTCCCCCGCAGAACGGTATCCCCGCGGAA; TACACGGCCCCTCATCCCCACCCCGCGCCAGAGTACACAGGCCAGACCACGGTTCCCGAG; CACACATTAAACCTGTACCCTCCCGCCCAGACGCACTCCGAGCAGAGCCCGGCGGACACG; AGCGCTCAGACCGTCTCTGGCACCGCCACACAGACAGATGACGCAGCACCGACGGATGGC; CAGCCCCAGACACAACCTTCTGAAAACACGGAAAACAAGTCTCAGCCCAAGCGGCTGCAT; GTCTCCAATATCCCCTTCAGGTTCCGGGATCCGGACCTCAGACAAATGTTTGGTCAATTT; GGTAAAATCTTAGATGTTGAAATTATTTTTAATGAGCGAGGCTCAAAGGGATTTGGTTTC; GTAACTTTCGAAAATAGTGCCGATGCGGACAGGGCGAGGGAGAAATTACACGGCACCGTG; GTAGAGGGCCGTAAAATCGAGGTAAATAATGCCACAGCACGTGTAATGACAAATAAAAAG; ACCGTCAACCCTTATACAAATGGCTGGAAATTGAATCCAGTTGTGGGTGCAGTCTACAGT; CCCGAATTCTATGCAGGCACGGTCCTGTTGTGCCAGGCCAACCAGGAGGGATCTTCCATG; TACAGTGCCCCCAGTTCACTTGTATATACTTCTGCAATGCCAGGCTTCCCGTATCCAGCA; GCCACCGCCGCGGCCGCCTACCGAGGGGCGCACCTGCGAGGCCGCGGTCGCACCGTGTAC; AACACCTTCAGGGCCGCGGCGCCCCCGCCCCCGATCCCGGCCTACGGCGGTGTTGTTTAC; CAGGATGGATTTTATGGTGCAGACATTTATGGTGGTTATGCTGCATACCGCTACGCCCAG; CCTACCCCTGCCACTGCCGCTGCCTACAGTGACAGTTACGGACGAGTTTATGCTGCCGAC; CCCTACCACCACGCACTTGCTCCAGCCCCCACCTACGGCGTTGGTGCCATGAATGCTTTT; GCACCTTTGACTGATGCCAAGACTAGGAGCCATGCTGATGATGTGGGTCTCGTTCTTTCT; TCATTGCAGGCTAGTATATACCGAGGGGGATACAACCGTTTTGCTCCATACTAA; ; Clone variation with respect to NM_018723.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018723 unedited; TTCAACAATTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCTCTTG; CTGGCCGTCTGGGTGCACACACCGCTCCCTCGATCACCCCAGCCCCCTTCCTGGTCTCCC; GAGCGCGGGGTTTGAAGGTCACCTCCTTTCCAGTCCCCGTGCGAGCCGCGCTGCCGCCGC; CTCCTCCAGCCAGAGTCGGTGGGACTGGCTGCGCTGCCCTGAAGTGGTTCTCCAAGCAGC; GCGGAGGGTGGCGGACGGCGGACGGAGCCCAGGGGCCGCGTCGGGTGGGGAAACCCGAAC; TCGCGGAGGGGAATCCCTCCCCCTCCGCCCCAGCCCCCCAGCAGCACCCGCGGTGGGGCG; GGGGCGCTCTGCCAGCCCCGGGAACAGCAGAGGCGGCGGCACTGGCTGGACCCACGCGCG; CGCCTCCGGGGCTGAAGAAGGAAGGAGTGAGCCGAGCCGAGCACCCCACATCTGGAGGGG; ACAGCCAGCCGTGGGCCCCGCCCCGGCGTCCGGAGCAGGAGAACTCCGAGCTTCTTGCCC; AGGCAGAGAGAGCAGGAGCGGACCGCGCGCCCGGGATTGAGAGTCCTTGCGCTCCAGACC; CCCACCCAGTGGCCGCCAGGGTCCCCGCCTGTCCGGACCCTCGCCGCGCCCAGGCAGGCG; CGCCAGGGCGGTTGCTGACCCTGCCGCGAAGTTGCGGACAGTGCGTGAGAAACCAGCACC; CCCTTTNCGCCGCCTCCAGCTTATGGTGAGTGTGGCTGGGGGTGCATAAAGCGCACCGGT; ATTTCGGGGTTCTGGGGCCCCATAACCTGACCGCAGCCGGGCTCCCCGGGAGTTCCTAGA; CACTGGTCCAAGAACTCATGCCAGTGGAACTTACACCTTCCTTGATCGGACTCGCCTTCA; AAATTAAAGCAGACTGCCATACCTGCGTGGAATAACAAAACN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018723 unedited; GGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTATTAGAATTTTTTT; TTTTTTTAGAGTTGCTAAAATGATGTACTACTGCATGTATTGCAATACTCAGGCCTCGGA; AAGCTTCCTTTCTCCCCACATTGGAAGGTTTTTATGGTTTTGTCATTTAGTATGGAGCAA; AACGGTTGTATCCCCCTCGGTATATACTAGCCTGCAATGAAGAAAGAACGAGACCCACAT; CATCAGCATGGCTCCTATTCTTGGCATCAGTCAAAGGTGCAAAAGCATTCATGGCACCAA; CGCCGTATGTGGGGGCTGGAGCAAGTGCGTGGTGGTAGGGGTCGGCAGCATAAACTCGTC; CGTAACTGTCACTGTAGGCAGCGGCAGTGGCAGGGGTAGGCTGGGCGTATCGGTATGCAG; CATAACCACCATAAATGTCTGCACCATAAAATCCATCCTGGTAAACAACACCGCCGTATG; CCGGGATCGGGGGCGGGGGCGCCGCGGCCCTGAAGGTGTTGTACACGGTGCGACCGCGGC; CTCGCAGGTGCGCCCCTCGGTAGGCGGCCGCGGCGGTGGCTGCTGGATACGGGAAGCCTG; GCATTGCAGAAGTATATACAAGTGAACTGGGGGCACTGTACATGGAAGATCCCTCCTGGT; TGGCCTGGCCAACAGGACCGTGCCTGATATGATTCGGGACTGTAGACTGCACCCACAACT; GGATTCAATTTCCAGCCATTGTATAAAGGNTTTGACGGTCTTTTTATTTGCATTACACGT; GCTGTGGCATTATTACCCTCGATTTTACGGCCTCTACCCCGNTGCCCGTGTAATTTCTCC; TTCGCCTGTTCGCATCGCACTATTTTTTGAAGTTACGATACCAAATCCCTTTGAGCCTCG; CTCATTAAAAATATTTCAACTNCTAAGATTTACCNNATGACAACATGNGNCGGAGGNCCG; ATCCCGAACCTAAGGGGGATATTGGAGACTGCANNCGCGGNCTGAACTTGNTTTTCAGTT; TAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_018723
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018723.2, NP_061193.2
• RefSeq Size: 2279 bp
• RefSeq ORF: 1194 bp
• Locus ID: 54715
• UniProt ID: Q9NWB1
• Cytogenetics: 16p13.3
• Domains: RRM
• Summary: The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (4) differs in the 5' UTR and lacks an in-frame section of the 3' coding region, compared to variant 1. This results in a shorter isoform (4) with an alternate N-terminus compared to isoform 1. Variants 4 and 6 both encode isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.