PUF60 (NM_078480) Human Untagged Clone

SKU SC110270

PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PUF60
• Synonyms: FIR; RoBPI; SIAHBP1; VRJS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_078480, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGACGGCGACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGG; CGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGG; GCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGGCC; AAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGC; AGCTCACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGAT; GGCGGCTCAGCGGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTG; GGGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACT; CCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCTT; GGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATAGGGCAG; GCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGC; ACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACT; GGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCC; CAAGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTCA; CACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCTGTGGCAGCTGC; TGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCGGTGCTGGGTACCCTGGGCACA; CCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTG; CCCAGGCACCTGGAGTCATCACAGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGT; GGGAGTGGTGAACCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAA; GAAGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCGG; GCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTGATGGTTCTGCG; CAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTGACAGAGGAGTGTGGCAAGTTC; GGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCA; AGATCTTTGTGGAGTTTTCCATAGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTT; TGCTGGCCGCAAGGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078480 unedited; TATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGACGGACGCA; AGATGGCGACGGCGACCATAGCTCTCGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGG; CGGCGGCGGCGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACT; CCATCAAGATGGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGC; CCGAGCAGCAGGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGA; GTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGG; CAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGATGGCGGCTCAGC; GGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGG; GGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGT; CCTGGGACTCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCG; AAGCTGCACAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCA; AGGTGGGCAGACCCAGCAACATANGGCANGCCCAGCCCATCATAGACCAGTTGGCTGAGG; AGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGCCCAGGACCTCTCAGACGATGA; CATCAAGAGCGTGTTTGTAGCCTTTGGCAAGACAAGTTCTGACACTGGCCCGGGACCCAC; ACTGGCAGCACAGGGCTACGCTTCATTGATACGAGAAGCCAGTCGTCCCAGAGCTTGGTC; TCCATGACCTCTTGACTGGTGCCAGTACTGCGGTGGGCAGGTGTACACCCCCATGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078480 unedited; TTGGACGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCCAGCAGCATCCG; CACCTTTATCCGCACTGTAGGCTGGGCTGGGCAGAGCGCGCCTGGCCCCGGGGACACCAC; TGTATCACTATAAAACCCAGAGGAAACAAGGAACAAGTGCAAGTCCGGGGAGAGGGACCA; CTGTCACGCAGAGAGGTCACTGTTATCAAAACGCTCCTGGTCGTACACTTCAGCCACCAC; CTTGCGGCCAGCAAACCAGCGGCCATTGAGGGCCTGGATGGCCTTATGAGTCTCAGAGGC; TATGGAAAACTCCACAAAGATCTTGACAATGATTTCTGCATCCTCCTCCTCGCCTTGTTT; CTCTTGGTAGATGATGACGCGGTTCACGGCCCCGAACTTGCCACACTCCTCTGTCACCTC; CCCTTCCAGGTCATCATCGATGTCCTTGGGGTCCACCATGTTGCGCAGAACCATCACTGT; AGACTCCTGCTTGCGGAGCAGCTTCTGCATCACCATGTGTCGGGCGCTACTGCCCGAGAT; GCTCATGTGCTCCTGCTCGCTCAGCATCTCTGGCCGCTCTGACTCGGGAAACAGCTCCTC; TTCTTCCTTCTCCTTCTTGGGCTCCAGGAGACCCAGCGTTGGAGGGCTGGCCAGGATGGG; GTTCACCACTCCCACCGAGGGGATGGTGACCGGGATAGGAGGACGGGCTGGGGTCACACC; TGTGATGACTCCANGTGCCTGGGCAGCCATGACAGCCTGGGGCANAGTGCCCCAGGGCTG; GGCCAGGGTCAGTGCTGGGGACACCAGTCCAAGTGGCCCAGGGTACCCAGCACCGCTGCT; TCGGCCACTGCTTTCTGAGCTGTGATCTTGGCAATGGCTGCANCANCTGCCACAACAACG; GCAGGTGGGAAGGCCCTCCAGCGTGCTGGTGTGAATAAGGGCATGGNGCGGGTGAANCCT; TTGCCAACCGCAGTACTGGGCCACCAGTTCAAAAAGTTCATGGAAAAACCACATCTTGGG; ACAACGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_078480
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_078480.1, NP_510965.1
• RefSeq Size: 1946 bp
• RefSeq ORF: 1680 bp
• Locus ID: 22827
• UniProt ID: Q9UHX1
• Cytogenetics: 8q24.3
• Domains: RRM, RRM_1
• Protein Pathways: Spliceosome
• Summary: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]; Transcript Variant: This variant (1) encodes the isoform a.