TBXAS1 (NM_001061) Human Untagged Clone
SKU
SC109823
TBXAS1 (untagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1
$498.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | TBXAS1 |
| Synonyms | BDPLT14; CYP5; CYP5A1; GHOSAL; THAS; TS; TXAS; TXS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001061 |
| Insert Size | 1960 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001061.2, NP_001052.1 |
| RefSeq Size | 1946 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 6916 |
| UniProt ID | P24557 |
| Cytogenetics | 7q34 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
| Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Aug 2008 Transcript Variant: This variant (1, also known as TXS-I) encodes isoform 1, which is also known as isoform TXS-I. Both variants 1 and 3 encode the same isoform. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is better supported by available conservation data and peptide data. This CCDS represents use of a downstream start codon, shortening the protein by a single amino acid. |
Reviews
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Resources
"NM_001061" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208028 | TBXAS1 (Myc-DDK-tagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1 | 10 ug |
$497.00
|
|
| RC208028L3 | Lenti ORF clone of Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$797.00
|
|
| RC208028L4 | Lenti ORF clone of Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1, mGFP tagged | 10 ug |
$797.00
|
|
| RG208028 | TBXAS1 (tGFP-tagged) - Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1 | 10 ug |
$489.00
$697.00
|
|
| SC322621 | TBXAS1 (untagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1 | 10 ug |
$498.00
|
|
Citations
Functional Role of the Conserved I-Helix Residue I346 in CYP5A1-Nanodiscs
Meling, Zelasko, Das
Biophysical Chemistry (2015)
Meling, Zelasko, Das
Biophysical Chemistry (2015)
AI Summary: OriGene contributed to this paper by providing the human CYP5A1 gene, which was used for cloning and subsequent functional studies.
Snippet: Materials. The human CYP5A1 gene was obtained from Origene. ... The CYP5A1 gene (Origene) was cloned into the pAr5 (modified pCWOri +) plasmid using the NdeI and XbaI restriction fragment sites located downstream of the IPTG-inducible tac promoter with Amp ... Cite SaveSaving
18β-Glycyrrhetinic Acid Suppresses Cell Proliferation through Inhibiting Thromboxane Synthase in Non-Small Cell Lung Cancer
Huang, Chu, Huang et al
PLoS ONE (2014) 9 (4), e93690 DOI: 10.1371/journal.pone.0093690
Huang, Chu, Huang et al
PLoS ONE (2014) 9 (4), e93690 DOI: 10.1371/journal.pone.0093690
Cited by 51
AI Summary: In this study, OriGene's pCMV6-TxAS plasmid was used to overexpress thromboxane synthase in NSCLC cells, enabling the researchers to demonstrate that 18β-glycyrrhetinic acid suppresses cell proliferation by inhibiting TxAS-driven signaling pathways.
Snippet: into A549 and NCI-H460, while 2 μg vacant pCMV6 (control) or pCMV6-TxAS plasmid was transfected into NCI-H23, with the aid of Lipofectamine 2000 reagents (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instruction (OriGene technologies, Rockville ... Cite SaveSaving
Functional Investigations of Thromboxane Synthase (CYP5A1) in Lipid Bilayers of Nanodiscs
Das, Varma, Mularczyk et al
Chembiochem (2014)
Das, Varma, Mularczyk et al
Chembiochem (2014)
Cited by 26
AI Summary: OriGene contributed to this study by providing the human CYP5A1 gene, which was essential for expressing and investigating the function of thromboxane synthase in lipid nanodiscs.
Snippet: Materials: The human CYP5A1 gene was obtained from Origene. Ampicillin, arabinose, chloramphenicol, isopropyl β-D-1-thiogalactopyranoside (IPTG), and Ni-NTA resin were purchased from Gold Biotechnology. δ-Aminolevulinic ... Cite SaveSaving
Showing 1-3 of 3 papers.
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