Caspase-7 (CASP7) (NM_033340) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Caspase-7 |
| Synonyms | CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_033340 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_033340.2, NP_203126.1 |
| RefSeq Size | 2433 bp |
| RefSeq ORF | 762 bp |
| Locus ID | 840 |
| UniProt ID | P55210 |
| Cytogenetics | 10q25.3 |
| Domains | Peptidase_C14 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease, Apoptosis |
| Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, May 2012 Transcript Variant: This variant (b, also known as beta) has multiple differences in the 5' UTR and coding region, and initiates translation at a downstream in-frame start codon, compared to variant d. The encoded isoform (beta) is shorter and has a distinct C-terminus, compared to isoform delta. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_033340" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221493 | CASP7 (Myc-DDK-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta | 10 ug |
$450.00
|
|
| RC221493L3 | Lenti-ORF clone of CASP7 (Myc-DDK-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta | 10 ug |
$750.00
|
|
| RC221493L4 | Lenti-ORF clone of CASP7 (mGFP-tagged)-Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta | 10 ug |
$750.00
|
|
| RG221493 | CASP7 (tGFP-tagged) - Human caspase 7, apoptosis-related cysteine peptidase (CASP7), transcript variant beta | 10 ug |
$650.00
|
|
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