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MCT2 (SLC16A7) (NM_004731) Human Untagged Clone

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SKU
SC108858
SLC16A7 (untagged)-Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCT2
Synonyms MCT2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC108858 sequence for NM_004731 edited (data generated by NextGen Sequencing)
ATGCCACCAATGCCAAGTGCCCCACCTGTGCATCCACCTCCAGATGGAGGATGGGGTTGG
ATTGTGGTTGGAGCAGCTTTTATCTCCATTGGATTTTCCTATGCATTCCCCAAAGCTGTC
ACCGTATTCTTCAAAGAAATTCAGCAAATATTCCACACTACCTACAGTGAAATAGCATGG
ATTTCATCCATTATGCTGGCTGTTATGTACGCAGGAGGTCCTGTAAGTAGTGTTTTGGTG
AATAAATACGGCAGCCGGCCGGTGGTGATAGCAGGAGGCTTATTATGCTGTCTTGGAATG
GTGTTGGCCTCCTTTAGTAGCAGCGTGGTACAGCTGTACCTCACTATGGGATTCATTACA
GGTTTAGGTTTAGCCTTCAACCTGCAACCCGCCTTAACCATAATTGGCAAATACTTCTAT
AGGAAGCGACCCATGGCAAATGGATTGGCCATGGCAGGAAGTCCTGTTTTCTTAAGTTCA
TTGGCTCCTTTCAATCAGTACCTTTTTAATACTTTTGGCTGGAAAGGAAGCTTCCTGATT
TTGGGAAGTCTACTTTTGAATGCCTGTGTGGCTGGTTCCCTCATGAGACCCCTTGGACCC
AATCAAACCACTTCTAAGTCTAAAAATAAGACTGGCAAAACAGAAGATGATTCAAGCCCA
AAGAAAATCAAAACGAAGAAATCAACTTGGGAAAAAGTTAATAAGTATTTAGATTTCTCC
CTTTTTAAGCATAGAGGATTTCTGATATATCTGTCTGGAAATGTCATTATGTTCCTAGGT
TTTTTTGCCCCCATTATATTCTTGGCTCCATATGCTAAAGACCAAGGAATTGATGAGTAC
TCGGCAGCTTTTCTGCTATCTGTTATGGCTTTCGTTGATATGTTTGCTAGGCCTTCTGTA
GGATTAATTGCAAACTCCAAATATATTCGACCTCGAATTCAGTACTTCTTCAGTTTTGCA
ATCATGTTCAATGGAGTGTGTCACCTCTTGTGCCCACTGGCACAGGACTACACAAGCCTG
GTATTATATGCTGTATTTTTTGGCCTTGGATTTGGGAGTGTTAGCAGTGTTCTCTTTGAA
ACTCTCATGGACCTCGTGGGTGCACCAAGATTTTCCAGTGCCGTCGGACTTGTCACAATT
GTGGAGTGTGGCCCAGTTCTTCTTGGCCCTCCTCTTGCAGGTAAATTGGTGGATTTAACT
GGAGAATATAAATACATGTACATGTCCTGTGGGGCTATTGTGGTAGCAGCAAGCGTGTGG
CTGCTCATTGGCAATGCTATCAACTATAGATTGCTTGCAAAGGAAAGGAAGGAGGAAAAT
GCAAGGCAGAAGACCAGAGAATCTGAACCCTTGAGCAAATCTAAACATTCGGAAGATGTT
AATGTCAAAGTTTCAAATGCACAGAGTGTAACCTCAGAAAGAGAAACTAACATTTAA

Clone variation with respect to NM_004731.3
1383 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004731 unedited
TTCACATTATGTAATACGTACTCACTATAGGGCGGCCGCGAATTCGCACGCAGGCCCACA
AGGGCGTTCAGCAGCCGCGCCTCCCCTTCCCGCCACCGCCTCCTTCTCCGCTGGCTGTGG
CGGGCGAGGACACGTCAGGGGCCATAAATAAATAAATAATTCCATTCACCCGTTGACAGC
GAGGCGAATCGGCTGCGGTTTGCTGGGAACGGAGGGCTGCAGCGCCCACCCTGCGCCAGA
GACCAGATAAAGAGTCAATCTTAAGATGTGATACTTTCCTGTGAAACCTGAAACAAGGTG
ATCTGGGGAACCAAAGACTCTGGGACTCTTGGTGCCAACAGAGTTACTCTGTTACTTGAA
TTTCCACTAGAGGAGCAGAAATGCCACCAATGCCAAGTGCCCCACCTGTGCATCCACCTC
CAGATGGAGGATGGGGTTGGATTGTGGTTGGAGCAGCTTTTATCTCCATTGGATTTTCCT
ATGCATTCCCCAAAGCTGTCACCGTATTCTTCAAAGAAATTCAGCAAATATTCCACACTA
CCTACAGTGAAATAGCATGGATTTCATCCATTATGCTGGCTGTTATGTACGCAGGAGGTC
CTGTAAGTAGTGTTTTGGTGAATAAATACGGCAGCCGGCCGGTGGTGATAGCAGGAGGCT
TATTATGCTGTCTTGGAATGGTGTTGGCCTCCTTTAGTAGCAGCGTGGTACAGCTGTACC
TCACTATNGGATTCATTACAGGTTTAGGGTTAGCCTTTAACCTGCAACCCGCCTTAANCC
ATTAATGNCAAAAACTTCTATAGGAAGCGAACCCTGGCAAATGGATTGGCCCATTGCCAG
AAGNCCTGTTTTCCTTAAGATCATTGGCTCCTTTCAATAAGAACCTCTTTATACTTTTGG
CTGAAAAGGAGCTTCCGATTTGGGAAGCCTACTTTTGAGCCTGGGCGGCTGGTCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004731 unedited
AGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAAATTA
TGATTGGAGAGGTACTACACAATTTAGAGGTTACATTTCTTGACAAATCTGAGTTGAATA
AAAAAGAACTAAAAATATAAACATAGCTAATTCAAGAATCAAGATCGGGTGAAACTAAAT
TAAAAACAGCCATAAAGTCCCACATTTTCCCTAATTTATTTACCTATGATATTGGCACAT
CTTGTTTTACTTAACATTTGAAAAATACAAATATCTCTTCATATGCAAAAGAGCAAATGT
TAAATGAAAAACTGAATTTTATATAGCTTCCTAAATATGAGCCACATGCTTAAGCAGTGA
ATTAACTTTATGAATCAAATGTTTAATGTTAACTATTACAAAAGCCTGGTATCCAGGTGC
AATCTCACCAGAAAAAAATAGCACCTGCCTTTATTACAATAAAAAAAATGGCTAATTCCC
TTTGACATCTTAAGAGTGACACTACTTTGTCAAGCACTAAATACTTTTTAAATTAATCAT
AATGTTAAACTTCAGATTTATAGATATAAATATTGTATTTATAACTTTAGTCATAATGTA
CAATTTCTTTCTTGTTTAAAAGTACTTTCTCACTCAAAATGGTATTTATAGTTTAAAAAG
AATATGCCTCTGTATTCAAGAGTGATTCAAATTCCCAGATGTTTTCACATAGTTATGAAA
ATGGGAAAGTGCTGGATTAAACTGGGCTGCAATATTCCAAGTTTTTGCTATCAGGTATAA
CTATACCTACGATTGAAGGCTACAGTGCCTCTATTTCTAGAAGCTTTTAAAGTTCCTGGC
TTANTATGTCCAAGGCGCAGGAAGCTCATCGTAGAGGCAGATNTTCAGGGCTGCCTTCTT
ATCCCAGAGGCTTTCCGGGTTGGAGCCAGGCCGGTGGAGTTGGCTTTGGCCTTGCTTTGG
G
Restriction Sites NotI-NotI |
ACCN NM_004731
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004731.3, NP_004722.2
RefSeq Size 3575 bp
RefSeq ORF 1437 bp
Locus ID 9194
UniProt ID O60669
Cytogenetics 12q14.1
Domains sugar_tr
Protein Families Transmembrane
Summary This gene is a member of the monocarboxylate transporter family. Members in this family transport metabolites, such as lactate, pyruvate, and ketone bodies. The protein encoded by this gene catalyzes the proton-linked transport of monocarboxylates and has the highest affinity for pyruvate. This protein has been reported to be more highly expressed in prostate and colorectal cancer specimens when compared to control specimens. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2012
Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_004731" in other vectors (6)
SKU Description Size Price
RC205361 SLC16A7 (Myc-DDK-tagged)-Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7) 10 ug
$289.00 $457.00
RC205361L1 Lenti ORF clone of Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7), Myc-DDK-tagged 10 ug
$757.00
RC205361L2 Lenti ORF clone of Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7), mGFP tagged 10 ug
$757.00
RC205361L3 Lenti ORF clone of Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7), Myc-DDK-tagged 10 ug
$757.00
RC205361L4 Lenti ORF clone of Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7), mGFP tagged 10 ug
$757.00
RG205361 SLC16A7 (tGFP-tagged) - Human solute carrier family 16, member 7 (monocarboxylic acid transporter 2) (SLC16A7) 10 ug
$489.00 $657.00

file-alt Citations

MCT2 mediates concentration-dependent inhibition of glutamine metabolism by MOG
Fets, Driscoll, Grimm et al
Nat. Chem. Biol. (2018) 14 (11), 1032-1042 DOI: 10.1038/s41589-018-0136-y
AI Summary: OriGene contributed to this study by providing the human MCT2 cDNA plasmid (clone ID SC108858, NM_004731), which was used to investigate the role of MCT2 in mediating MOG uptake and its effects on glutamine metabolism.
Snippet: Plasmids containing cDNAs of human MCTs were as follows: MCT2 was obtained from OriGene (clone ID SC108858, NM_004731) and cDNAs for MCT1 (Dharmacon MHS6278-202806621, BC026317.1) and MCT4 (MHS6278-211688899, BC112267.1)
Showing 1-1 of 1 papers.
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