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SH3BP4 (NM_014521) Human Untagged Clone

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SKU
SC108606
SH3BP4 (untagged)-Human SH3-domain binding protein 4 (SH3BP4)
  $883.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SH3BP4
Synonyms BOG25; TTP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC108606 sequence for NM_014521 edited (data generated by NextGen Sequencing)
ATGGCGGCTCAGCGGATCCGAGCGGCCAACTCCAATGGCCTCCCTCGCTGCAAGTCAGAG
GGGACCCTGATTGACCTGAGCGAAGGGTTTTCAGAGACGAGCTTTAATGACATCAAAGTG
CCTTCTCCCAGTGCCTTGCTCGTAGACAACCCCACACCTTTCGGAAATGCAAAGGAAGTG
ATTGCGATCAAGGACTATTGCCCCACCAACTTCACCACACTGAAGTTCTCCAAGGGCGAC
CATCTCTACGTCTTGGACACATCTGGCGGTGAGTGGTGGTACGCACACAACACCACCGAA
ATGGGCTACATCCCCTCCTCCTATGTGCAGCCCTTGAACTACCGGAACTCAACACTGAGT
GACAGCGGTATGATTGATAATCTTCCAGACAGCCCAGACGAGGTAGCCAAGGAGCTGGAG
CTGCTCGGGGGATGGACAGATGACAAAAAAGTACCAGGCAGAATGTACAGTAATAACCCT
TTCTGGAATGGGGTCCAGACCAATCCATTTCTGAATGGGAACGTGCCCGTCATGCCCAGC
CTGGATGAGCTGAATCCCAAAAGTACTGTGGATTTGCTCCTTTTTGACGCAGGTACATCC
TCCTTCACCGAATCCAGCTCAGCCACCACGAATAGCACTGGCAACATCTTCGATGAGCTT
CCAGTCACAAACGGACTCCACGCAGAGCCGCCGGTCAGGCGGGACAACCCCTTCTTCAGA
AGCAAGCGCTCCTACAGTCTCTCGGAACTCTCCGTCCTCCAAGCCAAGTCCGACGCTCCC
ACATCGTCGAGTTTCTTCACCGGCTTGAAATCACCTGCCCCCGAGCAATTTCAGAGCCGG
GAGGATTTTCGAACTGCCTGGCTAAACCACAGGAAGCTGGCCCGGTCTTGCCACGACCTG
GACTTGCTTGGCCAAAGCCCTGGTTGGGGCCAGACCCAAGCCGTGGAGACAAACATCGTG
TGCAAGCTGGATAGCTCCGGGGGTGCTGTCCAGCTTCCTGACACCAGCATCAGCATCCAC
GTGCCCGAGGGCCACGTCGCCCCTGGGGAGACCCAGCAGATCTCCATGAAAGCCCTGCTG
GACCCCCCGCTGGAGCTCAACAGTGACAGGTCCTGCAGCATCAGCCCTGTGCTGGAGGTC
AAGCTGAGCAACCTGGAGGTGAAAACCTCTATCATCTTGGAGATGAAAGTGTCAGCCGAG
ATAAAAAATGACCTTTTTAGCAAAAGCACAGTGGGCCTCCAGTGCCTGAGGAGCGACTCG
AAGGAAGGGCCATATGTCTCCGTCCCGCTCAACTGCAGCTGTGGGGACACGGTCCAGGCA
CAGCTGCACAACCTGGAGCCCTGTATGTACGTGGCTGTCGTGGCCCATGGCCCAAGCATC
CTCTACCCTTCCACCGTGTGGGACTTCATCAATAAAAAAGTCACAGTGGGTCTCTACGGC
CCTAAACACATCCACCCATCCTTCAAGACGGTAGTGACCATTTTTGGGCATGACTGTGCC
CCAAAGACGCTCCTGGTCAGCGAGGTCACACGCCAGGCACCCAACCCTGCCCCGGTGGCC
CTGCAGCTGTGGGGGAAGCACCAGTTCGTTTTGTCCAGGCCCCAGGATCTCAAGGTCTGT
ATGTTTTCCAATATGACGAATTACGAGGTCAAAGCCAGCGAGCAGGCCAAAGTGGTGCGA
GGATTCCAGCTGAAGCTGGGCAAGGTGAGCCGCCTGATCTTCCCCATCACCTCCCAGAAC
CCCAACGAGCTCTCTGACTTCACGCTGCGGGTTCAGGTGAAGGACGACCAGGAGGCCATC
CTCACCCAGTTTTGTGTCCAGACTCCTCAGCCACCCCCTAAAAGTGCCATCAAGCCTTCC
GGGCAAAGGAGGTTTCTCAAGAAGAACGAAGTCGGGAAAATCATCCTGTCCCCGTTTGCC
ACCACTACAAAGTACCCGACTTTCCAGGACCGCCCGGTGTCCAGCCTCAAGTTTGGTAAG
TTGCTCAAGACTGTGGTGCGGCAGAACAAGAACCACTACCTGCTGGAGTACAAGAAGGGC
GACGGGATCGCCCTGCTCAGCGAGGAGCGGGTCAGGCTCCGGGGCCAGCTGTGGACCAAG
GAGTGGTACATCGGCTACTACCAGGGCAGGGTGGGCCTCGTGCACACCAAGAACGTGCTG
GTGGTCGGCAGGGCCCGGCCCAGCCTGTGCTCGGGCCCCGAGCTGAGCACCTCGGTGCTG
CTGGAGCAGATCCTGCGGCCCTGCAAATTCCTCACGTACATCTATGCCTCCGTGAGGACC
CTGCTCATGGAGAACATCAGCAGCTGGCGCTCCTTCGCTGACGCCCTGGGCTACGTGAAC
CTGCCGCTCACCTTTTTCTGCCGGGCAGAGCTGGATAGTGAGCCCGAGCGGGTGGCGTCC
GTCCTGGAAAAGCTGAAGGAGGACTGTAACAACACTGAGAACAAAGAACGGAAGTCCTTC
CAGAAGGAGCTTGTGATGGCCCTACTGAAGATGGACTGCCAGGGCCTGGTGGTCAGACTC
ATCCAGGACTTTGTGCTCCTGACCACGGCTGTAGAGGTGGCCCAGCGCTGGCGGGAGCTG
GCTGAGAAGCTGGCCAAGGTCTCCAAGCAGCAGATGGACGCCTACGAGTCTCCCCACCGG
GACAGGAACGGGGTTGTGGACAGCGAGGCCATGTGGAAGCCTGCGTATGACTTCTTACTC
ACCTGGAGCCATCAGATCGGGGACAGCTACCGGGATGTCATCCAGGAGCTGCACCTGGGC
CTGGACAAGATGAAAAACCCCATCACCAAGCGCTGGAAGCACCTCACTGGGACTCTGATC
TTGGTGAACTCCCTGGACGTTCTGAGAGCAGCCGCCTTCAGCCCTGCGGACCAGGACGAC
TTCGTGATTTGA

Clone variation with respect to NM_014521.2
774 t=>c;2406 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014521 unedited
GTTCAGAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGTGAGTCCCGC
GGNAGCCGCGCGCGCCCCCGGCTGGGCCGAGCCGCTGGCCGACGAGCGGAGCCTCAGGAG
CCGGCGGGGACGCCATGCGAGCCAGGAAGAAACATATTGCCGAGTGGATGCCGCCGCGCA
GCGTGTTTGCTTGAGGCAGAAGCTTCAGCATCTGCTGGGATAACTGGAGGAAGAAATATG
AAGCCTTAGCGGCTTTACCCGGGAAGCGAGTTTCGAGATGGCGGCTCAGCGGATCCGAGC
GGCCAACTCCAATGGCCTCCCTCGCTGCAAGTCAGAGGGGACCCTGATTGACCTGAGCGA
AGGGTTTTCAGAGACGAGCTTTAATGACATCAAAGTGCCTTCTCCCAGTGCCTTGCTCGT
AGACAACCCCACACCTTTCGGAAATGCAAAGGAAGTGATTGCGATCAAGGACTATTGCCC
CACCAACTTCACCACACTGAAGTTCTCCAAGGGCGACCATCTCTACGTCTTGGACACATN
TGGCGGTGAGTGGGTGGTACGCACACAACACCACCGAAATGGGCTACATCCCCTCCTCCT
ATGTGCAGCCCTTGAACTACCGGAACTCAACACTGAGTGACAGCGGTATGATTGATAATC
TTCCAGACAGCCCAGACGAGGTAGCCAAGGAGCTGGAGCTGCTCGGGGGGGATGGACAGA
TGACANANAAGTACCAGGCAGAATGTACAGTAATACCCTTCTGGAAAAGGGGGTCCAGAC
CAATCCATTTCTGATGGGGGAACGTGCCCGTCAGCCCAGCCCTGGAAGAGCTGATCCCAA
AGTACTGGGGGGGGATTGCTCCTTTTGGAAGGCAGGTACATCCTTCTTTCACGGATCCAG
CTCAGCCCCACGAAAAGCACTGGCACATTTTTNAAGAGCTTTCAGTCACAAACGACTCCC
CCAAAACCCCCGGTATAGGGGGGACAACCCCCTTTTAAGAGCAGGCCTCTACGACTTTTG
GAACT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014521 unedited
GCTTGGCCAGGCCGCATCTATGATCGGTTTTTTTTTTTTTTTTTTAAAATATCCAAATAA
AATTTACTGCAACTTTTGTAGAATTTTATTTGTGCTACAAGACACGTTGCATAGGAAACT
ATTTAAAGCCCCTGAGGAAAAAATATCCATGGTTTAAGGTGCAACTGGTTTTGTTTCTTC
TTTGGGGAAAAGGTGATAGATGGTCTCTGGGAGAAATTATGGGGTGGAGTTGAGAAGCAC
AATCGAAGGTTATATGGTGGGATGATTGGCGAATTGTGTGTCCTGGTTCTTGCAGCATAA
AATAGCTAATGTTTGTTCTTTTTTTCAGTCCGTTGGTCTTCAGTATCTGAATAAGTAGCT
ATACAACGTTTCAGGCCAGTAAGGGAGTATACATTGATTATACTAAGGAAGTAACATGTT
TCAAGAAAGCAGTTTTAGAGAATGAGATTCGGGATCCCAAATGGTCCCTGACAACTGCAG
GCACACAGGAAGTGTCCTGGCACGTTGCTAAGGATCACCTCACTCTACTGATTTGCCAGT
ATCAGTCCTTCCTCCTGCTCCCTTCCAACACGCGACATCTACGACGCATTGTGAGATTCA
CACAGCATGTCTGCACCCTAAAGTTATTAATGCAACACTGTGTCAAGTTCCTGATTTTAT
GTGAGTCACATTTTCATTTTTACAAAATGCTCATCTTGCTCAGATGATCTTTACATTGTA
CAAAAAATGGTTTCATCTAAACTATGACTTCCTTCCGAGGAGACACAGGTACACCAGGAA
GGGAAGAGGGTTAAGCCCTAGATGAAATGAAATGCANAGTTTGGCAAATGGCAGCTGCCA
CCTCTGCACATCAGCATAAGGGCGTGCTTGTGAAGGCCACCACAGCATCTGCAATAAGCA
CGATAGTCACTGGCCACCAAGGCTCTGATCCGGNCATGAAGTTTCCTTCCTGTCCAAANA
TGAGCCAGCAACTCTATGGG
Restriction Sites ECoRI-NOT |
ACCN NM_014521
Insert Size 5150 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014521.1, NP_055336.1
RefSeq Size 5145 bp
RefSeq ORF 2892 bp
Locus ID 23677
UniProt ID Q9P0V3
Cytogenetics 2q37.2
Domains SH3
Protein Families Druggable Genome
Summary This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs, an SH3 domain, a PXXP motif, a bipartite nuclear targeting signal, and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis, specifically controlling the internalization of a specific protein receptor. provided by RefSeq, Jul 2008
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"NM_014521" in other vectors (6)
SKU Description Size Price
RC208730 SH3BP4 (Myc-DDK-tagged)-Human SH3-domain binding protein 4 (SH3BP4) 10 ug
$882.00
RC208730L1 Lenti ORF clone of Human SH3-domain binding protein 4 (SH3BP4), Myc-DDK-tagged 10 ug
$1,182.00
RC208730L2 Lenti ORF clone of Human SH3-domain binding protein 4 (SH3BP4), mGFP tagged 10 ug
$1,182.00
RC208730L3 Lenti ORF clone of Human SH3-domain binding protein 4 (SH3BP4), Myc-DDK-tagged 10 ug
$1,182.00
RC208730L4 Lenti ORF clone of Human SH3-domain binding protein 4 (SH3BP4), mGFP tagged 10 ug
$1,182.00
RG208730 SH3BP4 (tGFP-tagged) - Human SH3-domain binding protein 4 (SH3BP4) 10 ug
$1,082.00

file-alt Citations

DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma
Zhang, Tang, Liang et al
Oncogene (2020)
Cited by 42
AI Summary: OriGene products pCMV6-XL5-VPS35 (Cat# SC113646), pCMV6-XL4-SH3BP4 (Cat# SC108606), and pCMV6-Entry-PNO1 (Cat# RC203434) provided the full-length cDNAs of VPS35, SH3BP4, and PNO1, which were essential for validating the oncogenic functions of these genes in liver hepatocellular carcinoma in this study.
Snippet: Liver hepatocellular carcinoma (LIHC) is the second leading cause of cancer mortality worldwide. Although cancer driver genes identified so far have been considered to be saturated or nearly saturated, challenges remain in discovering novel genes underlying;The full-length cDNA of VPS35 (Accession No. NM_018206) was amplified from plasmid pCMV6-XL5-VPS35 (Cat# SC113646, OriGene, Rockville, MD), and inserted into the BamH I and Xho I sites of the shuttle vector pAdTrack-TO4 (from Dr T-C He, University of Chicago, Chicago, IL). The cDNAs of SH3BP4 (NM_014521) and PNO1 (NM_020143) were amplified from plasmid pCMV6-XL4-SH3BP4 (Cat# SC108606, OriGene) and pCMV6-Entry-PNO1 (Cat# RC203434),
Showing 1-1 of 1 papers.
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