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MRPL43 (NM_032112) Human Untagged Clone

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SKU
SC108410
MRPL43 (untagged)-Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1
  $150.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRPL43
Synonyms bMRP36a; L43mt; MRP-L43
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC108410 sequence for NM_032112 edited (data generated by NextGen Sequencing)
ATGACGGCGCGCGGGACTCCGAGCCGCTTCTTGGCCAGCGTTCTCCACAACGGACTGGGT
CGCTATGTGCAGCAGCTGCAGCGTCTGAGCTTCAGCGTCAGCCGCGACGGCGCCTCGTCT
CGCGGCGCCAGGGAGTTCGTGGAGCGGGAGGTGATCGACTTCGCCCGACGGAATCCAGGG
GTCGTAATATATGTAAACTCGCGTCCGTGCTGCGTGCCCAGAGTAGTGGCCGAATACCTT
AACGGGGCTGTGCGCGAGGAGAGCATCCACTGCAAGTCGGTCGAGGAGATCTCGACGCTG
GTGCAGAAGCTGGCCGACCAGTCGGGCTTGGACGTGATCCGCATCCGCAAGCCCTTCCAC
ACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCACCAACAAGCCGACCACGTTC
CGCGGGCTACGCCCCCGAGAGGTTCAGGATCCTGCCCCAGCCCAGGTGCAAGCACAGTGA

Clone variation with respect to NM_032112.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_032112 unedited
GTTAGNATTTGTATCCGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTAGGCTCC
GCGGCCTCCAAGCTGTAGCTATGACGGCGCGCGGGACTCCGAGCCGCTTCTTGGCCAGCG
TTCTCCACAACGGACTGGGTCGCTATGTGCAGCAGCTGCAGCGTCTGAGCTTCAGCGTCA
GCCGCGACGGCGCCTCGTCTCGCGGCGCCAGGGAGTTCGTGGAGCGGGAGGTGATCGACT
TCGCCCGACGGAATCCAGGGGTCGTAATATATGTAAACTCGCGTCCGTGCTGCGTGCCCA
GAGTAGTGGCCGAATACCTTAACGGGGCTGTGCGCGAGGAGAGCATCCACTGCAAGTCGG
TCGAGGAGATCTCGACGCTGGTGCAGAAGCTGGCCGACCAGTCGGGCTTGGACGTGATCC
GCATCCGCAAGCCCTTCCACACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCA
CCAACAAGCCGACCACGTTCCGCGGGCTACGCCCCCGAGAGGTTCAGGATCCTGCCCCAG
CCCAGGTGCAAGCACAGTGAAGAGTTGCCCCACCAACTGCAGCCCCAGGCTTTGGACTGT
TACTCCGGTAAAGGTGGTTCTTCCCCTTTGGGATTCCAAGCCCAGGCAAATGGAACCCAT
CAATGGGCAAGTTGACAGAGGTTCTGCTTGGGATAATGAAGAGCTGCCTGTTTCTTTCCA
GTGCCTGCTTCTGGGGGCAGTGACCTTGTGAACCACTCATTNTTATGCAAGTGGCATCCC
TAAACCTGAGATGAGGAGACTTCAAGGGTTTTACGGACCCTTGTTTTTAATCCANNATGA
TATATGATCTCNAAACANTGAGAGGTCTGAAGGCTGGCTCTGAGAATCCCTGATGTCTAT
TGGACACCCTGACTACGAAGCTTGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032112 unedited
AAAACCAAAGAAAAAAAAANNCNCCCCNNCCCCCCNTANTTTTGACTTTGANCCGCGGNC
CGCATNCNAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTGGCCTTGGGTAAGTTTATTAA
CCTGTTTTATAAATCTGTATTCACACAGATATAAAGTGCCTAGCCCATCACAGCAGAGCT
CTCCGTAGCTCAGTGGTTGTTCCAATAAGACATCAGGGATTCTTCAGAAGCCAGCCTTCA
GACCTCTCACTGTGTTTTGAGATCATTATTATCAATTTGGATTTAAAAAACAAGGGTCCT
GTAAAACCCTTGAAGTCTTCCTCATCTCAGGTTTTAGGGATGCCACTTGCATAAAAATGA
GTGGTTCACAAGGTCACTGCCCCCAGAAGCAGGCACTGGAAAGAAACAGGCAGCTCTTCA
TTATCCCAAGCAGAACCTCTGTCAACTTGCCCATTGATGGGTTCCATTTGCCTGGGCTTG
GAATCCCAAAGGGGAAGAACCACCTTTACCGGAGTAACAGCCCAAAGCCTGGGGCTGCAG
TTGGTGGGGCAACTCTTCACTGTGCTTGCACCTGGGCTGGGGCAGGATCCTGAACCTCTC
GGGGGCGTATTCCGCGGAACGTGGGCGGCTTGTTGGTGAAAGGGTGCCACTGGCCCTGAA
TGCCTAGGTTGTCGGTGTGGAAGGGCTTGCGGTTGCCGATTACGTTCAACCCTGACTGGT
CCGCCCTCTTCCTGCACCAGCTGTCAATACCCCCTCGACCGACTCCGCCTGGATGCCTCT
CCCTTGTCCCCCACCCCCCCTCAGGTGTTTCGGCCCCTTATTTTGGGTCCCCCCTCCGGT
CTCCAAGTTCCATAACTTACCTCCCCCCGGGTATCCCTGGGGGAAATCCGACAACCCCAC
CCTCCCCAACTTTTTGCTCCACTCACACTAGCCCCCTCCTTGT
Restriction Sites NotI-NotI |
ACCN NM_032112
Insert Size 1000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032112.2, NP_115488.2
RefSeq Size 1016 bp
RefSeq ORF 480 bp
Locus ID 84545
UniProt ID Q8N983
Cytogenetics 10q24.31
Domains L51_S25_CI-B8
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes isoform a.
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Documents
Resources
"NM_032112" in other vectors (4)
SKU Description Size Price
RC203936 MRPL43 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$150.00
RC203936L3 Lenti ORF clone of Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC203936L4 Lenti ORF clone of Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$450.00
RG203936 MRPL43 (tGFP-tagged) - Human mitochondrial ribosomal protein L43 (MRPL43), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00

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