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NELFE (NM_002904) Human Untagged Clone

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SKU
SC107909
NELFE (untagged)-Human RD RNA binding protein (RDBP)
  $686.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NELFE
Synonyms D6S45; NELF-E; RD; RDBP; RDP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_002904, the custom clone sequence may differ by one or more nucleotides


ATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCTGCAGAAGAAATTCAACAAGCTCAAGA
AAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAAAGTAGCAGCAGCACAACCAGCCAAGGTGGTGTCAA
ACGCTCACTATCAGAGCAGCCTGTCATGGACACAGCCACAGCAACAGAGCAGGCAAAGCAGCTGGTGAAG
TCAGGAGCCATCAGTGCCATCAAGGCTGAGACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGG
GGAAGTTAAAGGACCCCGAGAAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCTGCTGA
TGATGACCTGCAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAGCTTTGTGTCTTCTAGT
GATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGCCCAGGGGCTGGTGATGGTCCCCCTC
GAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGTGCCCACTCCTCAGCCTCCCCTCCCCGAAGCCGCAG
CCGGGACCGCAGCCATGAGAGGAACCGGGACAGAGACCGAGATCGGGAGCGGGATCGAGACCGGGATCGA
GACAGAGACAGAGAGCGGGACAGGGATCGGGATCGGGATCGAGATCGAGACCGGGAACGGGACAGGGATC
GGGAGCGGGATCGAGACCGAGACCGAGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCGAGC
CCCTAGGAAAGGGAATACTCTCTATGTATATGGAGAAGACATGACACCCACCCTTCTCCGTGGGGCCTTC
TCTCCTTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTCACCTATGAAA
AGATGGAGTCAGCAGATCAGGCCGTTGCTGAGCTCAACGGGACCCAGGTGGAGTCTGTACAGCTCAAAGT
CAACATAGCCCGAAAACAGCCCATGCTGGATGCCGCTACTGGCAAGTCTGTCTGGGGCTCCCTCGCTGTC
CAGAACAGCCCTAAGGGTTGCCACCGGGACAAGAGGACCCAGATTGTCTACAGTGATGACGTCTACAAGG
AAAACCTTGTGGATGGCTTCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_002904 unedited
AGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTGCCGCC
CCCTCGGGAGCCACCATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCTG
CAGAAGAAATTCAACAAGCTCAAGAAAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCAA
AGTAGCAGCAGCACAACCAGCCAAGGTGGTGTCAAACGCTCACTATCAGAGCAGCCTGTC
ATGGACACAGCCACAGCAACAGAGCAGGCAAAGCAGCTGGTGAAGTCAGGAGCCATCAGT
GCCATCAAGGCTGAGACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGGGGAAG
TTAAAGGACCCCGAGAAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATCT
GCTGATGATGACCTGCAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAGC
TTTGTGTCTTCTAGTGATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGC
CCAGGGGCTGGTGATGGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGT
GCCCACTCCTCAGCCTCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCCTGAGAGGAAC
CGGGACAGAGACCGAGATCGGGAGCGGGATCGAGACCGGGATCGAGACAGAGACAGAGAG
CGGGACAGGGATCGGGATCGGGATCGAGATCGAGACCGGGAACGGGACAGGGATCGGGAG
CGGGATCGAGACCGAGACCGAGAGGGTCCTTTCCGCNAGTCGGATTCATTCCCTGAACGG
CGAGCCNCCTAGAAAGGGAATACTCTCTATGTATATGGAGGAAGAATGACACCCACCCTC
CTCCGTGGGGCCTTTCTCTCCTTTTGGAAACATCATNGACCTCTCCATGGACCCACCCAN
AAACTGTGCCTTCGTACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002904 unedited
GGCTTCCTTGCCCGCGGCCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAG
CTTCCACCTCAGTGTTTTACTGAGACCAGCATTGCGGCATATGAGGCACAAGGAATCCAG
CTCTGTTCCCTAGAAGCCATCCACAAGGTTTTCCTTGTAGACGTCATCACTGTAGACAAT
CTGGGTCCTCTTGTCCCGGTGGCAACCCTTAGGGCTGTTCTGGACAGCGAGGGAGCCCCA
GACAGACTTGCCAGTAGCGGCATCCAGCATGGGCTGTTTTCGGGCTATGCTGACTTTGAG
CTGTACAGACTCCACCTGGGTCCCGTTGAGCTCAGCAACGGCCTGACCTGCTGACTCCAT
CTTTCCATAGGCGACGAAGGCACAGTTTCTGGGTGGGCCCATGGAGAGGCCAATGATGTT
CCCACCCGCACAGACCGCCCCACCGAGAACGGCGGCTGCCATGCCTTCTCCATATACATA
GACAGTATTCCCTTTCCTAGCGGCCCCCCCGTTCACGCAATCAATCCGACCTCGGCAAAG
GACCCTCTCGGTCTCCGTCTCGATCCCGCTCCCGATCCCTGCTCCGCCCCCCGGCTTGAT
CTCGATCCCGATCCCGATCCCTGTACCGCCCTCTGTCCCTGTCTCGATCCCCGCCTCGAT
CCCGTCCCGACCTTGGCCTCTGTCCCGGGCCCTCTCACGGGCTGCGGCCCCCGTTCGGGC
TCCCGCCAGGCGCCGTCCAGCATCGCCCCCCCTCGCCTCTTTCTACCCCCCGCCACACCT
CCACGGGACCCTCACCAGCCCCGTGGCCCCGGCCTTTCTCCATTGGGCCTCACTTTCCCC
ACGCCCCCCTGTACAACACCCGCCCTCCTCCCAACACCTCCCTTGCGGCCCCG
Restriction Sites NotI-NotI |
ACCN NM_002904
Insert Size 1270 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002904.5, NP_002895.3
RefSeq Size 1568 bp
RefSeq ORF 1143 bp
Locus ID 7936
UniProt ID P18615
Cytogenetics 6p21.33
Domains RRM
Protein Families Transcription Factors
Summary The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. provided by RefSeq, Jul 2008
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"NM_002904" in other vectors (7)
SKU Description Size Price
RC208658 NELFE (Myc-DDK-tagged)-Human RD RNA binding protein (RDBP) 10 ug
$732.00
RC208658L1 Lenti-ORF clone of NELFE (Myc-DDK-tagged)-Human RD RNA binding protein (RDBP) 10 ug
$1,032.00
RC208658L2 Lenti-ORF clone of NELFE (mGFP-tagged)-Human RD RNA binding protein (RDBP) 10 ug
$1,032.00
RC208658L3 Lenti-ORF clone of NELFE (Myc-DDK-tagged)-Human RD RNA binding protein (RDBP) 10 ug
$1,032.00
RC208658L4 Lenti-ORF clone of NELFE (mGFP-tagged)-Human RD RNA binding protein (RDBP) 10 ug
$1,032.00
RG208658 NELFE (tGFP-tagged) - Human RD RNA binding protein (RDBP) 10 ug
$932.00
SC324333 NELFE (untagged)-Human RD RNA binding protein (RDBP) 10 ug
$686.00

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