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PPP2R2B (NM_181674) Human Untagged Clone

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SKU
SC107323
PPP2R2B (untagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PPP2R2B
Synonyms B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B; PR55-BETA; PR55BETA; SCA12
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_181674, the custom clone sequence may differ by one or more nucleotides


ATGCTTCTCTCGTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCCACACA
GAAGGCTCGGACCATGGTGCAGTCCCACTGGCTCCCCTGCCCCCCTCTCCTGTGAGACTGGCTGCGGGGA
GGGATCATGGATACTTGTCTGCCGGCTTCTGGTTCCCACGCAAGTAAGCCTGCTGTCAATGGAGGAGGAC
ATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACCACAGCTATGCGACCGAAGCTGACATTATCT
CTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATT
TCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAG
AGCCATGAACCCGAGTTCGATTACCTGAAGAGTTTAGAAATAGAAGAAAAAATCAATAAAATAAGATGGC
TCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCTGTGGAAAGTCAG
CGAGCGTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGGGATCCTGCCACC
ATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCCCACGAAGAGTAT
TTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACAGCGACTATGAAACCTACATGTCCGC
TGATGACCTGAGGATTAACCTATGGAACTTTGAAATAACCAATCAAAGTTTTAATATTGTGGACATTAAG
CCAGCCAACATGGAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTCCACCCCCATCATTGCAACACCT
TCGTGTACAGCAGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCTGTGTGACAGGCA
CACCAAATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATTATCTCTTCGATT
TCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACTACTTGACCGTCAAAGTCTGGG
ATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACTACCTCCGCAGCAAGCTGTGTTC
CCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAATGGGTCAGACAGTGTCATCATG
ACAGGCTCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAGCGTGATGTGACCCTTGAGGCTT
CGAGGGAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGGCAAGCGGAGAAA
AGACGAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGGCATCCTTCAGAA
AATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACAAGGTTAACTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_181674 unedited
GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCGCTGATGGCA
AAATGGGACCCATCTCCAGTTATTCCTCGGGCACCCCAGCTTTGCAAATCTGGGAAAGAA
CCTACATAAACATATTGGTGATGTAGCCAGCTTAAAAGGCCGAGAGAATCCTTATGGATT
CAGGAGACTGTTTTTTTATTTAGATTTTTATTTTTTATTTGGAAGATGAAATGCTTCTCT
CGTTACCTGCCTTACATCTTCAGACCTCCGAACACCATCCTTTCTTCCAGCTGCCACACA
GAAGGCTCGGACCATGGTGCAGTCCCACTGGCTCCCCTGCCCCCCTCTCCTGTGAGACTG
GCTGCGGGGAGGGATCATGGATACTTGTCTGCCGGCTTCTGGTTCCCACGCAAGTAAGCC
TGCTGTCAATGGAGGAGGACATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACC
ACAGCTATGCGACCGAAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAAT
TACTAGCGACAGGGGACAAGGGGGGTCGGGTTGTAATATTTCAACGAGAGCAGGAGAGTA
AAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTACAGCACATTCCAGAGCCATGAAC
CCCGAGTTCGATTACCTCGAAGAGGTTTAGAAATAGAAGAGAAAATCAATAGAATAAGAT
GGCCTCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAANNACTGTGAA
CTGTGGGAAAGTCAGCGAGCGTGATTNAGAGCCCAGAAAGCTACATCTGAAGATAAAAAG
AGGGCCGCTCTCCGGTATCTGCCACATACACCCTTGCGGA
Restriction Sites NotI-NotI |
ACCN NM_181674
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181674.1, NP_858060.1
RefSeq Size 2284 bp
RefSeq ORF 1332 bp
Locus ID 5521
UniProt ID Q00005
Cytogenetics 5q32
Protein Families Druggable Genome, Phosphatase
Protein Pathways Tight junction
Summary The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. provided by RefSeq, Jul 2016
Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, compared to variant 3. The resulting isoform (a) is shorter and has a distinct N-terminus, compared to isoform e.
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Documents
Resources
"NM_181674" in other vectors (4)
SKU Description Size Price
RC224533 PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 10 ug
$457.00
RC224533L3 Lenti-ORF clone of PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 10 ug
$757.00
RC224533L4 Lenti-ORF clone of PPP2R2B (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 10 ug
$757.00
RG224533 PPP2R2B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 2 10 ug
$657.00

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