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PCDHGA5 (NM_032054) Human Untagged Clone

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SKU
SC106435
PCDHGA5 (untagged)-Human protocadherin gamma subfamily A, 5 (PCDHGA5), transcript variant 2
  $745.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PCDHGA5
Synonyms CDH-GAMMA-A5; ME3; PCDH-GAMMA-A5
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032054, the custom clone sequence may differ by one or more nucleotides


ATGGCGAGTCCACCTAGGGGCTGGGGCTGCGGAGAGCTGCTGCTGCCCTTCATGCTCCTGGGGACGCTGT
GCGAGCCAGGATCCGGGCAGATCCGCTACTCGATGCCGGAGGAGCTGGACAAAGGCTCCTTCGTCGGCAA
CATAGCCAAGGACCTTGGGCTGGAGCCCCAGGAGCTGGCGGAGCGCGGAGTCCGCATCGTCTCCAGAGGT
AGGACGCAGCTTTTTGCCCTGAACCCGCGAAGCGGCAGCTTGGTCACCGCGGGCAGGATAGACCGGGAGG
AGCTCTGCGCTCAGAGCCCACTGTGTGTGGTGAACTTTAACATCTTGGTTGAGAACAAAATGAAAATTTA
TGGAGTAGAAGTAGAAATAATCGATATTAATGATAACTTCCCGCGTTTCCGGGATGAAGAGTTAAAAGTA
AAAGTTAATGAAAATGCGGCTGCAGGGACACGGTTAGTGCTTCCCTTCGCGCGGGATGCGGATGTGGGTG
TGAACTCTCTCCGGAGTTACCAGCTCAGCTCCAATCTGCACTTCTCTCTGGATGTGGTAAGCGGAACTGA
TGGACAAAAGTATCCGGAGCTGGTGTTGGAACAGCCCCTAGACCGCGAGAAAGAGACTGTTCACGACCTC
CTCCTCACAGCTTTAGATGGCGGAGACCCGGTACTCTCCGGCACCACGCACATCCGTGTTACGGTCCTCG
ACGCAAACGACAATGCGCCCCTGTTCACCCCATCCGAGTACAGCGTGAGTGTTCCAGAGAACATACCTGT
GGGCACTCGGCTGCTCATGCTAACCGCCACGGATCCAGATGAGGGAATAAACGGGAAATTGACCTACTCT
TTTCGCAATGAAGAAGAAAAAATTTCGGAGACTTTCCAACTTGATTCCAACCTGGGGGAAATCTCAACTC
TACAATCACTGGACTATGAAGAATCCAGATTCTACCTCATGGAAGTGGTAGCTCAGGATGGAGGCGCTCT
TGTTGCCAGCGCTAAGGTGGTGGTCACAGTACAGGACGTGAATGACAATGCCCCCGAAGTGATCCTCACC
TCTCTGACCAGTTCGATCTCTGAAGACTGTCTTCCCGGAACTGTAATCGCGCTGTTTAGCGTACATGATG
GTGATTCTGGAGAAAATGGTGAGATTGCATGCTCTATTCCTAGGAATTTGCCTTTTAAATTGGAGAAGTC
AGTTGATAATTACTATCACCTATTAACAACTAGGGACCTGGACAGAGAAGAGACTTCAGATTATAATATC
ACTTTAACCGTCATGGACCATGGAACCCCGCCCCTCTCTACAGAAAGCCACATCCCCTTGAAAGTAGCAG
ACGTTAATGACAACCCACCCAATTTCCCTCAAGCCTCCTACTCCACCTCTGTCACAGAAAACAATCCCAG
AGGTGTCTCTATCTTCTCTGTGACAGCCCATGACCCCGACAGCGGCGACAACGCTCGAGTCACCTACTCC
CTGGCTGAAGACACATTTCAGGGGGCGCCCTTGTCCTCCTATGTATCCATTAACTCTGACACCGGTGTCC
TGTATGCTCTGAGATCCTTCGACTATGAGCAGTTGAGAGACCTACAGTTGTGGGTGACAGCCAGCGACAG
TGGGAACCCTCCACTTAGCAGCAACGTGTCGCTGAGCCTGTTTGTGCTGGACCAGAACGACAATACGCCT
GAGATCCTGTACCCCGCCCTCCCCACAGACGGTTCCACGGGCGTGGAGCTGGCGCCTCGCTCCGCAGAAC
CTGGCTACCTGGTGACCAAGGTGGTAGCGGTGGACAAAGATTCAGGCCAGAACGCCTGGCTGTCCTACCG
CCTGCTTAAGGCCAGCGAGCCAGGACTCTTTGCGGTTGGGCTGCACACGGGCGAGGTGCGCACAGCGCGA
GCCCTGCTGGACAGAGACGCGCTCAAGCAGAGCCTCGTGGTGGCCGTCGAAGACCATGGCCAGCCCCCTC
TGTCAGCCACCTTCACGGTCACCGTTGCCGTGGCCGACAGGATCCCTGACATCCTGGCTGACCTAGGCAG
TATCAAGACCCCCATTGACCCTGAGGATCTGGACCTCACACTCTATCTTGTGGTGGCAGTGGCTGCAGTC
TCCTGCGTCTTCCTGGCCTTCGTCATCGTGCTGCTGGTGCTCAGACTGAGGCGCTGGCACAAGTCACGCC
TGCTTCAGGCTGAAGGCAGCAGGTTGGCGGGTGTGCCCGCCTCGCACTTTGTGGGCGTGGATGGGGTTCG
GGCTTTCCTGCAGACCTATTCCCACGAGGTCTCCCTCACCGCGGACTCGAGGAAGAGTCACCTGATCTTT
CCCCAGCCCAACTACGCAGACACGCTCCTTAGTGAAGAGAGCTGTGAGAAAAGCGAGCCTCTTCTGATGT
CTGATAAGGTAGATGCAAACAAAGAAGAACGGCGAGTTCAGGTTAGTTTTCTCTTTCGGTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032054 unedited
GAATTCGGCACGAGGCAAGCTCCTACGAAAAGCCAACGCTCCACGCCCATTTTCGTCAGG
GAATATGTACCCATCGGCTTTAGATAAATAAGGAAACAGCAGGCTGAACCAGAACTAAGA
GAAAATTGGGCAGAGAGAAGGCAATGGCGAGTCCACCTAGGGGCTGGGGCTGCGGAGAGC
TGCTGCTGCCCTTCATGCTCCTGGGGACGCTGTGCGAGCCAGGATCCGGGCAGATCCGCT
ACTCGATGCCGGAGGAGCTGGACAAAGGCTCCTTCGTCGGCAACATAGCCAAGGACCTTG
GGCTGGAGCCCCAGGAGCTGGCGGAGCGCGGAGTCCGCATCGTCTCCAGAGGTAGGACGC
AGCTTTTTGCCCTGAACCCGCGAAGCGGCAGCTTGGTCACCGCGGGCAGGATAGACCGGG
AGGAGCTCTGCGCTCAGAGCCCACTTGTGTGTGGTGAACTTNTAACATCTTGGTTGAGAA
CAAAATGAAAATTTATNGGAGTAGAAGTAGAAATAATCGATATTAATGATAACTTCNCCG
CGTTTCCGGGATGAAAAGTTAAAAGTAAAAGTTAATGAAAATGCGGCTGCAGGGACACGG
GTTAGTGCTTCCCTTCGCGCGATACNGATTTTTNGGGGTGTGACTCTTTTCCGGAGTTAC
CAGCTCAGCTCCAATCTGCACTTCTTCTTGGGATTGTGGNAAGCNGGAACTGATGGGACA
AAGATCNGAGGCTTGTGGGTGTGGGAAACAGCCCCTAGACCGCGAAAAAAAAAACTGTTC
ACGACCTTCTCTCAAA
Restriction Sites NotI-NotI |
ACCN NM_032054
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032054.1, NP_114443.1
RefSeq Size 2442 bp
RefSeq ORF 2442 bp
Locus ID 56110
UniProt ID Q9Y5G8
Cytogenetics 5q31.3
Domains CA
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes the shorter isoform (2).
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Documents
Resources
"NM_032054" in other vectors (4)
SKU Description Size Price
RC211208 PCDHGA5 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 5 (PCDHGA5), transcript variant 2 10 ug
$744.00
RC211208L3 Lenti ORF clone of Human protocadherin gamma subfamily A, 5 (PCDHGA5), transcript variant 2, Myc-DDK-tagged 10 ug
$1,044.00
RC211208L4 Lenti ORF clone of Human protocadherin gamma subfamily A, 5 (PCDHGA5), transcript variant 2, mGFP tagged 10 ug
$1,044.00
RG211208 PCDHGA5 (tGFP-tagged) - Human protocadherin gamma subfamily A, 5 (PCDHGA5), transcript variant 2 10 ug
$944.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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