SP1 (NM_138473) Human Untagged Clone
$1,079.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | SP1 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_138473 |
| Insert Size | 7000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_138473.2, NP_612482.2 |
| RefSeq Size | 7667 bp |
| RefSeq ORF | 2358 bp |
| Locus ID | 6667 |
| UniProt ID | P08047, E4Z9M7 |
| Cytogenetics | 12q13.13 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Huntington's disease, TGF-beta signaling pathway |
| Summary | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2014 Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_138473" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209154 | SP1 (Myc-DDK-tagged)-Human Sp1 transcription factor (SP1), transcript variant 1 | 10 ug |
$1,078.00
|
|
| RC209154L1 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,378.00
|
|
| RC209154L2 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, mGFP tagged | 10 ug |
$1,378.00
|
|
| RC209154L3 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,378.00
|
|
| RC209154L4 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, mGFP tagged | 10 ug |
$1,378.00
|
|
| RG209154 | SP1 (tGFP-tagged) - Human Sp1 transcription factor (SP1), transcript variant 1 | 10 ug |
$1,278.00
|
|
Citations
Piperlongumine overcomes osimertinib resistance via governing ubiquitination-modulated Sp1 turnover
Wang, Wang, Liao et al
JCI Insight (2025) 10 (6) DOI: 10.1172/jci.insight.186165
Wang, Wang, Liao et al
JCI Insight (2025) 10 (6) DOI: 10.1172/jci.insight.186165
Cited by 6
AI Summary: OriGene products, specifically the Sp1 (SC101137) and RNF4 (RC207273) cDNA plasmids, were used in this study to investigate the molecular mechanisms by which piperlongumine overcomes osimertinib resistance in non-small cell lung cancer.
Snippet: Sp1 shRNA (#1, TRCN0000020444;#2, TRCN0000020445), and RNF4 shRNA (#1, TRCN0000017053;#2, TRCN0000017054), were purchased from GE Horizon. cDNA plasmids, including Sp1 (SC101137) and RNF4 (RC207273), were obtained from Origene
Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia
Nagel, Pommerenke, Meyer et al
PLoS One (2020) 15 (10), e0240120 DOI: 10.1371/journal.pone.0240120
Nagel, Pommerenke, Meyer et al
PLoS One (2020) 15 (10), e0240120 DOI: 10.1371/journal.pone.0240120
Cited by 9
AI Summary: OriGene contributed expression constructs for ELK1 (SC116858), ETS1 (SC125359), EPX (RC218959), HMX2 (SC303658), and SP1 (SC101137), which were used to investigate the roles of these genes in the regulation and function of HMX2 and HMX3 in acute myeloid leukemia.
Snippet: Qiagen (Hilden, Germany). Expression constructs for ELK1 (SC116858), ETS1 (SC125359), EPX (RC218959), HMX2 (SC303658) and SP1 (SC101137) were purchased from Origene (Wiesbaden, Germany). SiRNAs (80 pmol Cached
Bisdemethoxycurcumin Enhances the Sensitivity of Non-small Cell Lung Cancer Cells to Icotinib via Dual Induction of Autophagy and Apoptosis
Xiang, Jiang, Shu et al
Int. J. Biol. Sci. (2020) 16 (9), 1536-1550 DOI: 10.7150/ijbs.40042
Xiang, Jiang, Shu et al
Int. J. Biol. Sci. (2020) 16 (9), 1536-1550 DOI: 10.7150/ijbs.40042
Cited by 28
AI Summary: OriGene products contributed to this study by providing the Sp1 plasmid and control plasmid (PCMV6 XL8), which were used for transient transfection experiments to investigate the molecular mechanisms underlying the combined effects of bisdemethoxycurcumin and icotinib in non-small cell lung cancer cells.
Snippet: … Plasmid transient transfection The Sp1 and control plasmid DNA (PCMV6 XL8) used in this study were purchased from OriGene (Rockville, MD). …The efficacy of transfection (including siSp1, siSp3, siHDAC1, siHDAC2, siVDAC1 and siBeclin-1) were verified by immunoblot assay (Figure S1D-S1F). Plasmid transient transfection The Sp1 and control plasmid DNA (PCMV6 XL8) used in this study were purchased from OriGene (Rockville, MD). For transient transfection, 5 × 105 cells per well were seeded into 6-wells plates and transiently transfection with 2 μg of Sp1 plasmids using Lipofectamine 2000 transfection reagent (Invitrogen Calsbad, CA) according to the manufacturer's instructions. …
Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation
Li, Gao, Yang et al
Onco Targets Ther (2019) 12, 5907-5916 DOI: 10.2147/OTT.S208988
Li, Gao, Yang et al
Onco Targets Ther (2019) 12, 5907-5916 DOI: 10.2147/OTT.S208988
AI Summary: OriGene's SP1 overexpressing vector (SP1-Vector) and its negative control vector (Vector-NC) were used in this study to manipulate SP1 expression in cells, enabling the researchers to investigate the molecular mechanisms underlying estradiol-induced RSK4 inactivation in ER+ breast cancer.
Snippet: SP1 overexpressing vector (SP1-Vector) and its negative control vector (Vector-NC) were obtained from OriGene (Beijing, China), and were transfected into cells using Lipofectamine 2000 (Thermo Fisher Scientific, MA, USA) according to the manufacturer's
Investigation of the role of DNA methylation in the expression of ERBB2 in human myocardium
Quiñones-Lombraña, Blair, Blanco
Gene (2017)
Quiñones-Lombraña, Blair, Blanco
Gene (2017)
AI Summary: OriGene products contributed to this study by providing the Sp1 expression vector and the empty pCMV6-XL6 plasmid, which were used in co-transfection assays to investigate the regulation of ERBB2 expression in human myocardium.
Snippet: … In co-transfection assays, cells were transfected with 100 ng of ERBB2 reporter constructs, 10 ng of pRL-TK, and 150 ng of Sp1 expression vector or empty pCMV6-XL6 plasmid (OriGene, Rockville, MD). …In co-transfection assays, cells were transfected with 100 ng of ERBB2 reporter constructs, 10 ng of pRL-TK, and 150 ng of Sp1 expression vector or empty pCMV6-XL6 plasmid (OriGene, Rockville, MD). …
Inactivation of PI3-K/Akt and reduction of SP1 and p65 expression increase the effect of solamargine on suppressing EP4 expression in human lung cancer cells
Chen, Tang, Wu et al
J. Exp. Clin. Cancer Res. (2015) 34 (1), 154 DOI: 10.1186/s13046-015-0272-0
Chen, Tang, Wu et al
J. Exp. Clin. Cancer Res. (2015) 34 (1), 154 DOI: 10.1186/s13046-015-0272-0
Cited by 43
AI Summary: OriGene products contributed to this study by providing the overexpression vectors pCMV6-AC-Akt, pCMV6-AC-SP1, and pCMV6-AC-EP4, which were used in transient transfection assays to investigate the roles of these genes in solamargine's suppression of EP4 expression in human lung cancer cells.
Snippet: Flow Systems). Transient transfection assay. The control, Akt, SP1, and EP4 overexpression vectors (pCMV6-AC-Akt, pCMV6-AC-SP1, pCMV6-AC-EP4) were obtained from OriGene Technologies, Inc. (Rockville, MD, USA). The ... Cite SaveSaving
Showing 1-6 of 21 papers.
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