EXOC6 (NM_019053) Human Untagged Clone

SKU SC100885

EXOC6 (untagged)-Human exocyst complex component 6 (EXOC6), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EXOC6
• Synonyms: EXOC6A; SEC15; SEC15L; SEC15L1; SEC15L3; Sec15p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100885 sequence for NM_019053 edited (data generated by NextGen Sequencing); ATGGCGGAGAACAGCGAGAGTCTGGGCACCGTCCCCGAGCACGAGCGGATCTTGCAGGAG; ATCGAGAGCACCGACACCGCCTGTGTGGGGCCCACCCTCCGGTCTGTGTATGATGACCAA; CCAAATGCGCACAAGAAGTTTATGGAAAAGTTAGATGCTTGTATCCGTAATCATGACAAG; GAAATTGAAAAGATGTGTAATTTTCATCATCAGGGTTTTGTAGATGCTATTACAGAACTC; CTTAAAGTAAGGACTGATGCAGAAAAACTGAAGGTGCAAGTTACTGATACCAACCGAAGG; TTTCAAGATGCTGGAAAAGAGGTGATAGTCCACACAGAAGATATCATTCGATGTAGAATT; CAGCAGAGAAATATTACAACTGTAGTAGAAAAATTGCAGTTATGCCTTCCTGTGCTAGAA; ATGTACAGTAAGCTGAAAGAACAGATGAGTGCCAAAAGGTACTATTCTGCCCTAAAAACT; ATGGAACAATTAGAGAATGTGTACTTTCCCTGGGTTAGTCAATACCGGTTTTGTCAGCTC; ATGATAGAAAATCTTCCCAAACTCCGTGAGGATATTAAAGAAATCTCCATGTCTGATCTC; AAAGACTTTTTGGAAAGTATTCGAAAACATTCTGACAAAATAGGTGAAACAGCAATGAAA; CAGGCACAGCATCAGAAAACCTTCAGTGTTTCTCTGCAGAAACAAAATAAAATGAAATTT; GGGAAAAATATGTATATAAATCGTGATAGAATTCCAGAGGAAAGGAATGAAACTGTATTG; AAACATTCACTTGAAGAAGAGGATGAGAATGAAGAAGAGATCTTAACTGTTCAGGATCTT; GTTGATTTTTCCCCTGTTTATCGATGTTTGCACATTTATTCTGTTTTGGGTGACGAGGAA; ACATTTGAAAACTATTATCGAAAACAAAGAAAGAAACAAGCAAGACTGGTATTGCAACCC; CAGTCGAATATGCATGAAACAGTTGATGGCTATAGAAGATATTTCACTCAAATTGTAGGG; TTCTTTGTGGTAGAAGATCACATTTTACATGTGACCCAAGGATTAGTAACCAGGGCATAC; ACTGATGAACTTTGGAACATGGCCCTCTCAAAGATAATTGCTGTCCTTAGAGCTCATTCA; TCCTATTGCACTGATCCTGATCTTGTTCTGGAGCTGAAGAATCTTATTGTAATATTTGCA; GATACTTTACAGGGTTATGGTTTTCCAGTGAACCGACTTTTTGACCTTTTATTTGAAATA; AGAGACCAATACAATGAAACACTGCTTAAGAAATGGGCTGGAGTTTTCAGGGACATTTTT; GAAGAAGATAATTACAGCCCCATCCCTGTTGTCAATGAAGAAGAATATAAAATTGTCATC; AGCAAATTTCCCTTTCAAGATCCAGACCTTGAAAAGCAGTCTTTCCCAAAGAAATTCCCC; ATGTCTCAGTCAGTGCCTCATATTTACATTCAAGTTAAAGAATTTATTTATGCCAGCCTT; AAATTTTCAGAGTCACTACACCGGAGCTCAACAGAAATAGACGATATGCTTAGAAAATCA; ACAAATCTGCTGCTGACCAGAACTTTGAGTAGCTGTTTACTGAACCTTATTAGAAAACCT; CATATAGGTTTGACAGAGCTGGTACAAATCATCATAAACACAACACACCTGGAGCAAGCT; TGTAAATATCTTGAGGACTTTATAACTAACATTACAAATATTTCCCAAGAAACTGTTCAT; ACTACAAGACTTTATGGACTTTCTACTTTCAAGGATGCTCGACATGCAGCAGAAGGAGAA; ATATATACCAAACTGAATCAAAAAATTGATGAATTTGTTCAGCTTGCTGATTATGACTGG; ACAATGTCTGAGCCAGATGGAAGAGCTAGTGGTTATTTAATGGACCTTATAAATTTTTTG; AGAAGCATCTTTCAAGTGTTTACTCATTTGCCTGGGAAAGTTGCTCAGACAGCTTGCATG; TCAGCCTGCCAGCATCTGTCAACATCCTTAATGCAGATGCTACTGGACAGTGAGTTAAAA; CAAATAAGCATGGGAGCTGTTCAGCAGTTTAACTTAGATGTCATACAGTGTGAATTGTTT; GCCAGCTCTGAGCCTGTGCCAGGATTCCAGGGGGATACCCTGCAGCTAGCATTCATTGAC; CTCAGACAACTCCTTGACCTGTTTATGGTTTGGGATTGGTCTACTTACCTAGCTGATTAT; GGGCAGCCAGCTTCTAAGTACCTTCGGGTGAATCCAAACACAGCCCTTACTCTTTTGGAG; AAGATGAAGGATACTAGCAAAAAGAACAATATATTTGCTCAGTTCAGGAAGAATGATCGA; GACAAACAGAAGTTGATAGAGACAGTCGTGAAACAGCTGAGAAGTTTGGTGAATGGTATG; TCCCAGCACATGTAG; ; Clone variation with respect to NM_019053.4; 1187 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019053 unedited; TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGCTTCCACCA; AAATGGCGGAGAACAGCGAGAGTCTGGGCACCGTCCCCGAGCACGAGCGGATCTTGCAGG; AGATCGAGAGCACCGACACCGCCTGTGTGGGGCCCACCCTCCGGTCTGTGTATGATGACC; AACCAAATGCGCACAAGAAGTTTATGGAAAAGTTAGATGCTTGTATCCGTAATCATGACA; AGGAAATTGAAAAGATGTGTAATTTTCATCATCAGGGTTTTGTAGATGCTATTACAGAAC; TCCTTAAAGTAAGGACTGATGCAGAAAAACTGAAGGTGCAAGTTACTGATACCAACCGAA; GGTTTCAAGATGCTGGAAAAGAGGTGATAGTCCACACAGAAGATATCATTCGATGTAGAA; TTCAGCAGAGAAATATTACAACTGTAGTAGAAAAATTGCAGTTATGCCTTCCTGTGCTAG; AAATGTACAGTAAGCTGAAAGAACAGNATGAGTGCCAAAAGGTACTATTCTGCCCTAAAA; ACTATGGAACAATTAGAGAATGTGTACTTTCCCTGGGTTAGTCAATACCGGTTTTGTCAG; CTCATGATAGAAAATCTTCCCAAACTCCGTGAGGATATTAAAGAAATCTCCATGTCTGAT; CTCAAAGACTTTTTGGAAAGTATTCGAAAACATTCTGACAAAATAGGTGAAACAGCAATG; AAACAGGCACAGCATCAGAAAACCTTCAGTGTTTCTCTGCAGAAACAAATAAATGAAATT; TGGGAAAAAATGTATATAAATCGTGATAGAATTCCAGAGGAAGGAAATGAACTGTATTGA; AACATCACTTNGAGAAGAGATGAGATGAAGAAAGATCTTACTGTTCAGGGATCTGTGATT; TTNCCCTGTNATCGATGTTGCACATTATCTGNNTTGGNTGACAGGAAACATTGAAACTTT; NATCGAACAAGAAAGACN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019053 unedited; TAGCTTTGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAACAAC; TTGCTCAATATTTTTAATATTTCATCACAAAATCCAGAGATGTACATATAATTCTTTACA; CTTATTTTACAGAATCAGGCATAGTTTCTTACAGGCCTAAATCCTGAAAAGGCAAAGGGA; AGATTCAGCATAAATACAACAAAGGGAGAATAAAGACTGGATTTATATTGCCATTTGGAC; CACGGAAACATACACAAAGGAAAATGTACATTTGTTTTAGGTTTTCCAGAGTTCATTTGG; TTTGGCTTGTTTCCACAATGAGGATAAAGTACTGAAATCTGCTATTTGAAAAAAGTAACC; TGACCCCTGTGGTAACTTCTGTGTGCAGGAACCATCTACCCTCGCACAAGCTGCACCTGA; AGTGGTTAGCCCCATTTAGTTCCTGTCCTCTCTTTTAACTGGAACCAAACCAAACCAAAC; AAATCCAGAATGGGGTCACCTACAAGCAATATAGTTAGAAATATTAACCCTGATGAACAG; AAACAACACTGCATCTTTAAAAAATCCAAAACCTTTTGTTCATATTCAGAACGATTAAAT; TAAACCACCCAAGTAGAACAAATAATTCACATTCTTACTGAGTTAAATCAAGTTTGATTT; CAGGCAGCTTTTAAAATTTGTCTATTTCCCCTTCAGAATTAGCAGAATTAGAAATTTTGG; TCTGCTTAAATCACATTGAGGTGAAAATAAAAGCTAAAAGTCAGAAAATGGTAGTGAAAT; ACTACCATATCAGACATGATTGAATTCCAAGCCATATACTGATCAAAAACAGATTATCAT; TCAAGTCTAAATAGAAAAAATACTTATGAGTAGAGGCAATATGTTTTCAAATGTATTTGT; AATCAATTCAGTATAANTTGTGACATTATGTATTACACTTTGCATTTTCCCCTAAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_019053
• Insert Size: 3650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019053.3, NP_061926.3
• RefSeq Size: 3606 bp
• RefSeq ORF: 2415 bp
• Locus ID: 54536
• UniProt ID: Q8TAG9
• Cytogenetics: 10q23.33
• Domains: Sec15
• Summary: The protein encoded by this gene is highly similar to the Saccharomyces cerevisiae SEC15 gene product, which is essential for vesicular traffic from the Golgi apparatus to the cell surface in yeast. It is one of the components of a multiprotein complex required for exocytosis. The 5' portion of this gene and two neighboring cytochrome p450 genes are included in a deletion that results in an autosomal-dominant form of nonsyndromic optic nerve aplasia (ONA). Alternative splicing and the use of alternative promoters results in multiple transcript variants. A paralogous gene encoding a similar protein is present on chromosome 2. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) lacks two 5' exons but instead contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and 5' coding region, compared to variant 3. The encoded isoform (a) has a distinct and shorter N-terminus, compared to isoform c.