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TDRD7 (NM_014290) Human Untagged Clone

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SKU
SC100746
TDRD7 (untagged)-Human tudor domain containing 7 (TDRD7)
  $957.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TDRD7
Synonyms CATC4; PCTAIRE2BP; TRAP
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC100746 sequence for NM_014290 edited (data generated by NextGen Sequencing)
ATGCTGGAAGGAGATCTGGTTTCAAAGATGCTGCGAGCTGTTCTGCAGTCTCATAAGAAT
GGAGTAGCATTACCCCGGCTCCAAGGAGAGTACAGATCCTTGACTGGAGACTGGATCCCC
TTCAAACAGCTAGGTTTCCCTACACTAGAAGCCTATCTGAGAAGTGTGCCAGCAGTGGTC
AGGATAGAGACTAGTAGATCTGGAGAGATTACCTGCTATGCCATGGCCTGCACAGAAACT
GCAAGAATTGCTCAGCTTGTGGCTCGTCAAAGGAGTTCTAAAAGGAAAACCGGGCGTCAA
GTTAATTGTCAGATGAGAGTGAAGAAAACCATGCCATTTTTTCTAGAAGGAAAACCAAAA
GCAACCCTCAGACAACCAGGATTTGCTTCAAATTTTTCTGTTGGCAAAAAACCTAATCCA
GCACCGTTAAGAGACAAAGGAAACTCTGCTGGAGTTAAGCCTGATGCTGAAATGTCTCCT
TATATGCTACACACAACTCTTGGAAATGAAGCATTCAAAGACATTCCAGTGCAAAGGCAT
GTGACCATGTCCACCAACAACAGGTTTAGCCCAAAGGCGTCCCTTCAGCCACCTTTGCAG
ATGCATCTCTCAAGAACCTCTACTAAGGAAATGAGTGATAATTTAAATCAGACTGTTGAA
AAACCCAATGTCAAGCCTCCTGCCTCTTACACTTATAAAATGGATGAGGTTCAAAATCGC
ATAAAGGAAATACTAAACAAGCATAACAATGGCATTTGGATATCTAAGCTTCCACATTTT
TACAAAGAGTTATATAAAGAAGACCTTAATCAAGGAATTTTACAACAGTTTGAACACTGG
CCTCATATTTGCACGGTGGAGAAACCTTGCAGTGGTGGCCAAGATTTACTTCTTTATCCA
GCTAAGAGAAAGCAGCTTTTGAGAAGTGAACTGGATACTGAGAAAGTACCTCTATCCCCA
CTACCTGGTCCCAAACAAACACCACCGTTGAAAGGGTGTCCAACAGTTATGGCAGGAGAC
TTTAAAGAAAAAGTGGCAGACCTGCTGGTGAAATACACAAGTGGCCTTTGGGCCAGTGCA
CTTCCGAAAGCATTTGAGGAAATGTACAAAGTGAAATTCCCTGAGGATGCCTTAAAAAAT
CTTGCCTCACTTTCTGATGTATGCAGCATAGACTACATTTCTGGAAATCCCCAGAAGGCC
ATTCTCTATGCTAAACTTCCATTGCCCACTGACAAAATCCAAAAGGATGCAGGGCAAGCA
CATGGTGATAATGATATCAAGGCTATGGTTGAACAAGAGTATTTGCAGGTAGAAGAAAGC
ATTGCTGAAAGTGCTAATACCTTTATGGAGGACATAACAGTTCCTCCTTTAATGATTCCA
ACTGAAGCATCACCATCTGTATTGGTGGTTGAACTGAGCAACACAAATGAAGTGGTTATC
AGGTATGTGGGCAAAGACTATTCTGCTGCTCAGGAATTAATGGAAGATGAGATGAAGGAA
TATTACAGTAAGAATCCTAAGATCACACCAGTCCAGGCTGTGAATGTTGGGCAGTTGCTG
GCCGTAAATGCCGAGGAGGACGCCTGGTTACGGGCACAGGTCATCTCAACAGAAGAGAAC
AAAATAAAGGTATGCTATGTTGACTATGGTTTTAGTGAAAATGTTGAAAAAAGCAAAGCA
TACAAATTAAACCCGAAGTTTTGTTCACTCTCATTTCAAGCTACAAAATGTAAGCTTGCA
GGCTTGGAAGTCCTAAGCGATGACCCTGATCTAGTGAAGGTGGTTGAATCTTTAACTTGT
GGAAAGATCTTTGCAGTGGAAATACTTGACAAAGCTGACATTCCACTTGTTGTTCTGTAC
GATACCTCAGGAGAAGATGATATCAATATCAATGCCACCTGCTTGAAGGCTATATGTGAC
AAGTCACTAGAGGTTCACCTGCAGGTTGACGCCATGTACACAAATGTCAAAGTAACTAAT
ATTTGCTCTGATGGGACACTCTACTGCCAGGTGCCTTGTAAGGGTCTGAACAAGCTCAGT
GACCTTCTACGTAAGATAGAGGACTACTTCCATTGCAAGCACATGACCTCTGAGTGCTTT
GTTTCATTACCCTTCTGTGGGAAAATCTGCCTCTTCCATTGCAAAGGAAAATGGTTACGA
GTAGAGATCACAAATGTTCACAGCAGCCGGGCTCTTGATGTTCAGTTCCTGGACTCTGGC
ACTGTGACATCTGTAAAAGTGTCAGAGCTCAGGGAAATTCCACCTCGGTTTCTACAAGAA
ATGATTGCAATACCACCTCAGGCCATTAAGTGCTGTTTAGCAGATCTTCCACAATCTATT
GGCATGTGGACACCAGATGCAGTGCTGTGGTTAAGAGATTCTGTTTTGAATTGCTCGGAC
TGTAGCATTAAGGTTACAAAAGTGGATGAAACCAGAGGGATCGCACATGTTTATTTATTT
ACCCCTAAGAACTTCCCTGACCCTCATCGCAGTATTAATCGCCAGATTACAAATGCAGAC
TTGTGGAAGCATCAGAAGGATGTGTTTTTGAGTGCCATATCCAGTGGAGCTGACTCTCCC
AACAGCAAAAATGGCAACATGCCCATGTCGGGCAACACTGGAGAGAATTTCAGAAAGAAC
CTCACAGATGTCATCAAAAAGTCCATGGTGGACCATACGAGCGCTTTCTCCACAGAGGAA
CTGCCACCTCCTGTCCACTTATCAAAGCCAGGGGAACACATGGATGTGTATGTGCCTGTG
GCCTGTCACCCAGGCTACTTCGTCATCCAGCCTTGGCAGGAGATACATAAGTTGGAAGTT
CTGATGGAAGAGATGATTCTATATTACAGCGTGTCTGAAGAGCGCCACATAGCAGTGGAG
AAAGACCAAGTGTATGCTGCAAAAGTGGAAAATAAGTGGCACAGGGTGCTTTTAAAAGGA
ATCCTGACCAATGGACTGGTATCTGTGTATGAGCTGGATTATGGCAAACACGAATTAGTC
AACATAAGAAAAGTACAGCCCCTAGTGGACATGTTCCGAAAGCTGCCCTTCCAAGCAGTC
ACAGCTCAACTTGCAGGAGTGAAGTGCAACCAGTGGTCTGAGGAGGCTTCTATGGTGTTT
CGAAATCATGTGGAGAAGAAACCTCTGGTGGCACTGGTGCAGACAGTCATTGAAAATGCT
AACCCTTGGGACCGGAAAGTAGTGGTCTACTTAGTGGACACATCGTTGCCAGACACCGAT
ACCTGGATTCATGATTTTATGTCAGAGTATCTGATAGAGCTTTCAAAAGTTAATTAA

Clone variation with respect to NM_014290.2
33 a=>g;449 t=>c;588 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014290 unedited
CGAATTCGGCACCAGGTGGGCGGCGGCCGCGGCGGGGCCCCTGGCGGAGACGGCGGCAGG
AGCTGGGCCCAGAGACGCGGGGACGGGCCGTGGGCCCCCGGAACGAGGCAAAGATGCTGG
AAGGAGATCTGGTTTCAAAGATGCTGCGAGCTGTTCTGCAGTCTCATAAGAATGGAGTAG
CATTACCCCGGCTCCAAGGAGAGTACAGATCCTTGACTGGAGACTGGATCCCCTTCAAAC
AGCTAGGTTTCCCTACACTAGAAGCCTATCTGAGAAGTGTGCCAGCAGTGGTCAGGATAG
AGACTAGTAGATCTGGAGAGATTACCTGCTATGCCATGGCCTGCACAGAAACTGCAAGAA
TTGCTCAGCTTGTGGCTCGTCAAAGGAGTTCTAAAAGGAAAACCGGGCGTCAAGTTAATT
GTCAGATGAGAGTGAAGAAAACCATGCCATTTTTTCTAGAAGGAAAACCAAAAGCAACCC
TCAGACAACCAGGATTTGCTTCAAATTTTTCTGTTGGCAAAAAACCTAATCCAGCACCGT
TAAGAGACAAAGGAAACTCTGCTGGAGTTAAGCCTGATGCTGAAATGTCTCCTTATATGC
TACACACACCTCTTGGAAATGAAGCATTCAAAGACATTCCAGTGCAAAGGCATGTGACCA
TGTCCACCAACAACAGGTTTAGCCCAAAGGCGTCCCTTCAACCACCTTTGCAGATGCATC
TCTCAAGACCCTCTACTAAGGAAATGAGTGATACTTTAATA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014290 unedited
NNNNNCCGATAAAGCATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTT
TTTATATTGATAAACATTTTTATTCTAATATTATAGTAATAATTCAAGTACAATTTTCTT
TGAAAAGTCAACTCTGTTAAAACAAAACTTGTTAAATATCTTTCATCAAACATAAGCATA
TTCTTTTCAATCCCCCACAGCAGTCAGAGCCATGTAGCAGAGTTAAGATTTTTTTTAAGC
CTACTACATTTTGTTATTGCTAAAAAATCTGAATTAGTTGTCAAGGTTTCAGAGGCAGTC
ATTAATTAACTTTTGAAAGCTCTATCAGATACTCTGACATAAAATCATGAATCCAGGTAT
CGGTGTCTGGCAACGATGTGTCCACTAAGTAGACCACTACTTTCCGGTCCCAAGGGTTAG
CATTTTCAATGACTGTCTGCACCAGTGCCACCAGAGGTTTCTTCTCCACATGATTTCGAA
ACACCATAGAAGCCTCCTCAGACCACTGGTTGCACTTCACTCCTGCAAGTTGAGCTGTGA
CTGCTTGGAAGGGCAGCTTTCGGAACATGTCCACTAGGGGCTGTACTTTTCTTATGTTGA
CTAATTCGTGTTTGCCATAATCCAGCTCATACACAGATACCAGTCCATTGGTCAGGATTC
CTTTTAAAAGCACCCTGTGCCACTTATTTTCCACTTTTGCAGCATACACTTTGGTCTTTC
TCCACTGCTATGTGGCGCTCTTCAGACACGCTGGTATATAGGATCATCTCTTCCATCAGA
ACTTCCNACTTATGTATCNTCTGCNCAGGCTGGATGACGAAGTACCCTGGGTGACAGGCC
ACAGGCACATACACATCCATGTGTTCCCCTGGCTTGGATAATGGACAGGAGGTGGCAT
Restriction Sites NotI-NotI |
ACCN NM_014290
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014290.1, NP_055105.1
RefSeq Size 3784 bp
RefSeq ORF 3297 bp
Locus ID 23424
UniProt ID Q8NHU6
Cytogenetics 9q22.33
Domains TUDOR
Summary The protein encoded by this gene belongs to the Tudor family of proteins. This protein contains conserved Tudor domains and LOTUS domains. It is a component of RNA granules, which function in RNA processing. Mutations in this gene have been associated with cataract formation in mouse and human. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_014290" in other vectors (6)
SKU Description Size Price
RC205411 TDRD7 (Myc-DDK-tagged)-Human tudor domain containing 7 (TDRD7) 10 ug
$956.00
RC205411L1 Lenti ORF clone of Human tudor domain containing 7 (TDRD7), Myc-DDK-tagged 10 ug
$1,256.00
RC205411L2 Lenti ORF clone of Human tudor domain containing 7 (TDRD7), mGFP tagged 10 ug
$1,256.00
RC205411L3 Lenti ORF clone of Human tudor domain containing 7 (TDRD7), Myc-DDK-tagged 10 ug
$1,256.00
RC205411L4 Lenti ORF clone of Human tudor domain containing 7 (TDRD7), mGFP tagged 10 ug
$1,256.00
RG205411 TDRD7 (tGFP-tagged) - Human tudor domain containing 7 (TDRD7) 10 ug
$1,156.00

file-alt Citations

A new mechanism of interferon's antiviral action: Induction of autophagy, essential for paramyxovirus replication, is inhibited by the interferon stimulated gene, TDRD7
Subramanian, Kuzmanovic, Zhang et al
PLoS Pathog. (2018) 14 (1), e1006877 DOI: 10.1371/journal.ppat.1006877
AI Summary: OriGene's human and mouse TDRD7/Tdrd7 expression vectors were used to study the antiviral role of TDRD7 in inhibiting autophagy and paramyxovirus replication in this paper.
Snippet: taining 10% FBS, penicillin and streptomycin. All cell lines used in this study were maintained in the laboratory. Expression vectors of human and mouse TDRD7/Tdrd7 gene (untagged) were obtained from Origene and sub-cloned into lentiviral vector (pLVX-IRES-puro, V5-tagged). AMPK expression plasmid was obtained from Addgene and was sub-cloned into lentiviral vector (pLVX-IRES-puro, HA-tagged AMPK)
FigureFigureFigure
Showing 1-1 of 1 papers.
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