MARCHF8 (NM_145021) Human Untagged Clone

SKU SC100050

41341 (untagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 8

Product Data

• Type: Human Untagged Clone
• Target Symbol: MARCHF8
• Synonyms: c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145021, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAA; GTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAG; CAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACT; TCTATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGCCCCCTGA; TCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTGCAGCAGTGGATCAAGAG; CTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATGGAGACCAAGCTGAAGCCACTGAGA; AAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCA; TTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGG; GCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTGGTGGTTGTGGCCATCGGCTTCACCGGA; GGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAATTGTGGAAGAGACTCAAGGCCTATAATA; GAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAGAATATTTTTGAAAAATCTCCACTAACAGA; GCCCAACTTTGAAAATAAACATGGATATGGAATCTGTCATTCCGACACAAACTCTTCTTGTTGCACAGAG; CCTGAAGACACTGGAGCAGAAATCATTCACGTCTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145021 unedited; NGGGTTATTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA; TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC; GGCCGCGAATTCGGCACGAGGGTTGGGATGAGCATGCCACTGCATCAGATCTCTGCCATT; CCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAAGTAAGACCAAAGAAAAGGAGAGG; GAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAGCAACATTTCTAAG; GCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACTTCT; ATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGC; CCCCTGATCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTG; CAGCAGTGGATCAAGAGCTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATC; ATGGAGACCAAGCTGAAGCCACTGAGAAAATGGGAGAAGTTGCAGATGACGTCCAGCGAG; CGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCATTGCCATCACATGTGTGGTCTGG; TCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGGGCAGGCAACAGGA; ATCCTATAAGGGCCCTTTGGACAAATTGGTGGGTGTGGCCATCGGCTTCACCGGAGGACT; TCTTTTTATGTATGTTCACTGCAAAGCGCATGTGCCATTGTCGGAAAAGACCCAGGCCCT; TTATAGAAAGGACTATTGTTCAAAACCGTCCCGAAACAACCAAAAAGAATATTTTTCGAA; AAACTCCACCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145021 unedited; TCNAACGTAATCTATGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTAAAAAAAGGCTTTATT; GGTGCAATTAAACATCCCAGGGTATAGCACAAACCCCCCTGTACAAAAAATTATTCAAGG; GGTAATACTGAAAAACAGTGAACCCCCCCAAAAAAATACAAAACTAGCCATTTAGATCAC; TAAAAACTTTTTAAGGGAAAAAAAATTTCAAATGGGAAGGGCCTTTTAAAAACTACCCCC; CACATGCCAGGGGAAATTTGGTTTAACAATCAATTTTTATAAACATTGGATTTAACTGGG; CACTGATAAACTGGACAATTTTAAAAATTACTTCCAAGACAAAAATAAATGCTTTTATTT; CCCTATGTTTTGGGCTTTCCTTGGGCTAAAAACAAAAGCTGCATGTTTCAAACCCAATTT; CAAAGGGAAAGCCCTTTAAAACAAAAAGGACTTCAATTGGGTTTTTGCCTTTAGCCACAT; CCAAAGACTTGGGGATCTCACCCGGGTGGTCCCAACCCTTTTTCAACCCTGAAACCCAAG; CAGGGGGGCAAAGGCCCCCCCCAATTGTGCAAACATTCACCGAGGTTCCAAAATGGCCCA; ACTGGCTTGGGCCTGTTTGCCTGTGGGCCAGTTAGGAACAATGTGCCGGTTTGAAAAACA; AGGCCCCCCAAACTGGAAAAGTGGCGCGAAACCACAAGGGGGGAAAAAATTTGAGGCGCT; CACGGGGGGAAAGAACCCTTTTATAGAGTTTTCCGGAAAATCTTACCAAAAAATTGGGCC; ACAAAACAAATTTTTGGAGAGCAAAAACCCTCTTTATGGTACCGGGGGATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_145021
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145021.4, NP_659458.2
• RefSeq Size: 2511 bp
• RefSeq ORF: 876 bp
• Locus ID: 220972
• UniProt ID: Q5T0T0
• Cytogenetics: 10q11.21-q11.22
• Domains: RING
• Protein Families: Druggable Genome, Transmembrane
• Summary: MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]; Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 3' coding region, compared to variant 4. This results in a shorter protein (isoform a), compared to isoform b. Variants 2 and 3 encode the same isoform (a).