Human APLF (NM_173545) AAV Particle

SKU RC207936A1V

AAV ORF Particles, serotype AAV-2, APLF (Myc-DDK-tagged)-Human aprataxin and PNKP like factor (APLF), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: APLF
• Synonyms: APFL; C2orf13; PALF; Xip1; ZCCHH1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207936 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCCGGGGGCTTCGAGCTGCAGCCGCGGGACGGCGGTCCCCGGGTGGCCCTGGCGCCCGGGGAGACGG; TGATCGGCCGCGGGCCGCTGCTGGGAATAACAGACAAGAGAGTATCCAGAAGACATGCCATTCTTGAGGT; GGCAGGTGGTCAGCTGCGAATCAAACCGATACACACAAATCCATGTTTTTACCAGTCTTCAGAGAAGAGT; CAGCTCTTACCATTGAAGCCAAATCTATGGTGCTATTTGAATCCTGGAGACAGCTTTTCTTTGTTAGTTG; ACAAATACATTTTCCGCATTCTCTCTATACCCTCTGAAGTGGAAATGCAATGTACCTTAAGAAACAGTCA; AGTGCTTGATGAAGATAATATATTGAATGAAACACCAAAATCCCCCGTGATTAATTTACCTCATGAGACT; ACTGGTGCCTCACAACTGGAAGGAAGCACAGAAATAGCCAAGACCCAGATGACTCCCACAAATAGTGTGT; CTTTCCTAGGTGAAAATAGAGACTGCAATAAGCAGCAGCCAATCCTTGCCGAGAGGAAAAGAATCCTTCC; AACTTGGATGTTAGCAGAACATTTAAGTGATCAAAACCTTTCAGTACCAGCAATCAGTGGAGGTAATGTA; ATCCAGGGAAGTGGAAAAGAAGAAATCTGCAAAGATAAATCCCAGCTAAACACAACCCAGCAAGGAAGAA; GGCAATTAATTTCATCAGGAAGTTCAGAAAATACATCAGCAGAACAAGACACAGGAGAAGAGTGCAAAAA; TACTGATCAGGAAGAGTCTACCATTTCATCCAAGGAAATGCCACAATCATTTTCTGCAATCACATTAAGT; AACACAGAGATGAATAATATTAAGACTAATGCACAGAGAAACAAACTTCCAATAGAGGAACTTGGTAAAG; TTTCTAAACATAAAATTGCCACTAAAAGAACACCACATAAAGAAGATGAGGCAATGAGCTGTTCTGAAAA; TTGTTCGAGTGCCCAGGGCGACTCACTTCAGGATGAGTCTCAAGGGTCTCATTCTGAGTCCAGCTCTAAT; CCCTCCAATCCTGAAACTTTGCATGCAAAGGCAACTGATTCAGTTCTACAAGGTTCTGAAGGAAACAAGG; TCAAGAGGACATCCTGCATGTATGGGGCAAACTGCTATAGGAAGAATCCTGTTCATTTTCAACATTTTAG; CCATCCTGGTGATAGTGATTATGGAGGTGTACAAATCGTGGGCCAAGATGAGACTGATGACCGGCCTGAA; TGTCCCTATGGACCATCCTGTTATAGGAAGAATCCCCAGCACAAGATAGAATATAGACATAATACGCTTC; CAGTGAGAAATGTTTTAGATGAAGATAATGATAATGTTGGGCAACCCAATGAGTATGACCTGAACGACAG; CTTTCTAGATGATGAGGAAGAAGACTATGAGCCAACAGATGAAGATTCTGACTGGGAACCAGGAAAGGAA; GATGAAGAGAAGGAAGATGTGGAAGAGCTTTTGAAAGAAGCAAAAAGGTTTATGAAAAGAAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207936 protein sequence; Red=Cloning site Green=Tags(s); ; MSGGFELQPRDGGPRVALAPGETVIGRGPLLGITDKRVSRRHAILEVAGGQLRIKPIHTNPCFYQSSEKS; QLLPLKPNLWCYLNPGDSFSLLVDKYIFRILSIPSEVEMQCTLRNSQVLDEDNILNETPKSPVINLPHET; TGASQLEGSTEIAKTQMTPTNSVSFLGENRDCNKQQPILAERKRILPTWMLAEHLSDQNLSVPAISGGNV; IQGSGKEEICKDKSQLNTTQQGRRQLISSGSSENTSAEQDTGEECKNTDQEESTISSKEMPQSFSAITLS; NTEMNNIKTNAQRNKLPIEELGKVSKHKIATKRTPHKEDEAMSCSENCSSAQGDSLQDESQGSHSESSSN; PSNPETLHAKATDSVLQGSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPE; CPYGPSCYRKNPQHKIEYRHNTLPVRNVLDEDNDNVGQPNEYDLNDSFLDDEEEDYEPTDEDSDWEPGKE; DEEKEDVEELLKEAKRFMKRK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_173545
• ORF Size: 1533 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_173545.1
• RefSeq Size: 3869 bp
• RefSeq ORF: 1536 bp
• Locus ID: 200558
• UniProt ID: Q8IW19
• Cytogenetics: 2p13.3
• MW: 57 kDa