Human DGKA (NM_201444) AAV Particle

SKU RC202930A1V

AAV ORF Particle, Serotype AAV-2, DGKA (Myc-DDK-tagged)-Human diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: DGKA
• Synonyms: DAGK; DAGK1; DGK-alpha
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202930 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCAAGGAGAGGGGCCTAATAAGCCCCAGTGATTTTGCCCAGCTGCAAAAATACATGGAATACTCCA; CCAAAAAGGTCAGTGATGTCCTAAAGCTCTTCGAGGATGGCGAGATGGCTAAATATGTCCAAGGAGATGC; CATTGGGTACGAGGGATTCCAGCAATTCCTGAAAATCTATCTCGAAGTGGATAATGTTCCCAGACACCTA; AGCCTGGCACTGTTTCAATCCTTTGAGACTGGTCACTGCTTAAATGAGACAAATGTGACAAAAGATGTGG; TGTGTCTCAATGATGTTTCCTGCTACTTTTCCCTTCTGGAGGGTGGTCGGCCAGAAGACAAGTTAGAATT; CACCTTCAAGCTGTACGACACGGACAGAAATGGGATCCTGGACAGCTCAGAAGTGGACAAAATTATCCTA; CAGATGATGCGAGTGGCTGAATACCTGGATTGGGATGTGTCTGAGCTGAGGCCGATTCTTCAGGAGATGA; TGAAAGAGATTGACTATGATGGCAGTGGCTCTGTCTCTCAAGCTGAGTGGGTCCGGGCTGGGGCCACCAC; CGTGCCACTGCTAGTGCTGCTGGGTCTGGAGATGACTCTGAAGGACGACGGACAGCACATGTGGAGGCCC; AAGAGGTTCCCCAGACCAGTCTACTGCAATCTGTGCGAGTCAAGCATTGGTCTTGGCAAACAGGGACTGA; GCTGTAACCTCTGTAAGTACACTGTTCACGACCAGTGTGCCATGAAAGCCCTGCCTTGTGAAGTCAGCAC; CTATGCCAAGTCTCGGAAGGACATTGGTGTCCAATCACATGTGTGGGTGCGAGGAGGCTGTGAGTCCGGG; CGCTGCGACCGCTGTCAGAAAAAGATCCGGATCTACCACAGTCTGACCGGGCTGCATTGTGTATGGTGCC; ACCTAGAGATCCACGATGACTGCCTGCAAGCGGTGGGCCATGAGTGTGACTGTGGGCTGCTCCGGGATCA; CATCCTGCCTCCATCTTCCATCTATCCCAGTGTCCTGGCCTCTGGACCGGATCGTAAAAATAGCAAAACA; AGCCAGAAGACCATGGATGATTTAAATTTGAGCACCTCTGAGGCTCTGCGGATTGACCCTGTTCCTAACA; CCCACCCACTTCTCGTCTTTGTCAATCCTAAGAGTGGCGGGAAGCAGGGGCAGAGGGTGCTCTGGAAGTT; CCAGTATATATTAAACCCTCGACAGGTGTTCAACCTCCTAAAGGATGGTCCTGAGATAGGGCTCCGATTA; TTCAAGGATGTTCCTGATAGCCGGATTTTGGTGTGTGGTGGAGACGGCACAGTAGGCTGGATTCTAGAGA; CCATTGACAAAGCTAACTTGCCAGTTTTGCCTCCTGTTGCTGTGTTGCCCCTGGGTACTGGAAATGATCT; GGCTCGATGCCTAAGATGGGGAGGAGGTTATGAAGGACAGAATCTGGCAAAGATCCTCAAGGATTTAGAG; ATGAGTAAAGTGGTACATATGGATCGATGGTCTGTGGAGGTGATACCTCAACAAACTGAAGAAAAAAGTG; ACCCAGTCCCCTTTCAAATCATCAATAACTACTTCTCTATTGGCGTGGATGCCTCTATTGCTCATCGATT; CCACATCATGCGAGAGAAATATCCGGAGAAGTTCAACAGCAGAATGAAGAACAAGCTATGGTACTTCGAA; TTTGCCACATCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAGGAGTCTTTGACAGTTGAGATCTGTG; GGAAACCGCTGGATCTGAGCAACCTGTCCCTAGAAGGCATCGCAGTGCTAAACATCCCTAGCATGCATGG; TGGCTCCAACCTCTGGGGTGATACCAGGAGACCCCATGGGGATATCTATGGGATCAACCAGGCCTTAGGT; GCTACAGCTAAAGTCATCACCGACCCTGATATCCTGAAAACCTGTGTACCAGACCTAAGTGACAAGAGAC; TGGAAGTGGTTGGGCTGGAGGGTGCAATTGAGATGGGCCAAATCTATACCAAGCTCAAGAATGCTGGACG; TCGGCTGGCCAAGTGCTCTGAGATCACCTTCCACACCACAAAAACCCTTCCCATGCAAATTGACGGAGAA; CCCTGGATGCAGACGCCCTGTACAATCAAGATCACCCACAAGAACCAGATGCCCATGCTCATGGGCCCAC; CCCCCCGCTCCACCAATTTCTTTGGCTTCTTGAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202930 protein sequence; Red=Cloning site Green=Tags(s); ; MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIYLEVDNVPRHL; SLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDKIIL; QMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEWVRAGATTVPLLVLLGLEMTLKDDGQHMWRP; KRFPRPVYCNLCESSIGLGKQGLSCNLCKYTVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESG; RCDRCQKKIRIYHSLTGLHCVWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKT; SQKTMDDLNLSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRL; FKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQNLAKILKDLE; MSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFE; FATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTRRPHGDIYGINQALG; ATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGE; PWMQTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_201444
• ORF Size: 2205 bp
• Buffer: PBS with 0.001% Pluronic F5382
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_201444.2, NP_958852.1
• RefSeq Size: 2801 bp
• RefSeq ORF: 2208 bp
• Locus ID: 1606
• UniProt ID: P23743
• Cytogenetics: 12q13.2
• MW: 82.6 kDa