Avidin-Biotin Blocking Kit

SKU
E08-18
This product is designed to eliminate the non-specific binding caused by the endogenous avidin or biotin like substances in immunohistochemistry and cytochemistry, when either avidin or Streptavidin/biotin is used as the signal amplification system.
  $100.00
2 Weeks*
Specifications
Specifications
Product Data
Components Avidiin Solution
Biotin Solution
Coverslip No
Permanent No
Shipping Blue Ice
Description This product is designed to eliminate the non-specific binding caused by the endogenous avidin or biotin like substances in immunohistochemistry and cytochemistry, when either avidin or Streptavidin/biotin is used as the signal amplification system.
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Resources

file-alt Citations

The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells
Claude-Taupin, Isnard, Bagattin et al
Nat Commun (2023) 14 (1), 8056 DOI: 10.1038/s41467-023-43775-1
Cited by 55
AI Summary: In this study, the OriGene Avidin/Biotin Blocking Kit (E08-18) was used to prepare tissue sections for YAP protein detection, thereby facilitating the investigation of YAP/TAZ's role in regulating autophagy in kidney epithelial cells under different fluid flow conditions.
Snippet: 200 dilution) or SQSTM1 (PROGEN, GP62-C, 1:100) antibody overnight at 4 °C, followed with the appropriate secondary antibody. For YAP detection, sections were first treated with avidin/biotin kit (Origene, E08-18) before using YAP primary antibody (Cell Signaling Technology, #14074, 1:200) overnight at 4 °C. Sections were then incubated with biotinylated anti-rabbit antibody (GEHealthcare, R
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Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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