3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
0.156-10ng/mL
Sample Type
Human serum, plasma, cell lysates, tissue homogenates and other biological fluids.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of HTT
Sensitivity
57pg/mL
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
HTT Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
HTT Standard Protein | 0.1 mL
HTT Detection Antibody | 0.12 mL
1x SA-HRP Conjugate | 0.12 mL
Standard Diluent | 10 mL
Assay Buffer | 15 mL
Sample Diluent | 30 mL
Detection Antibody Diluent | 15 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 14 mL
Stop Solution | 14 mL
Background
Huntingtin (HTT) is the protein coded for in humans by the HTT gene. The function of HTT is not well understood but it is involved in axonal transport. HTT is a scaffolding protein in the ATM oxidative DNA damage response complex. Mutant HTT plays a key role in mitochondrial dysfunction involving the inhibition of mitochondrial electron transport, higher levels of reactive oxygen species and increased oxidative stress. Mutated HTT is the cause of Huntington's disease (HD), and has been investigated for this role and also for its involvement in long-term memory storage.
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in shipping.