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Human Chk2 (CHEK2) ELISA Kit 1 x 96

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SKU
EA200062
Human Chk2 (CHEK2) ELISA Kit 1 x 96
  $610.00
4 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich
Assay Length 3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal Colorimetric
Curve Range 78-5000pg/ml
Sample Type Human tissue homogenates, cell lysate and other biological fluids.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of CHEK2
Sensitivity 18.97pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • CHEK2 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • Human CHEK2 Standard (250ng/ml) | 0.1 mL
  • 100x Biotin conjugated CHEK2 Detection Antibody | 0.12 mL
  • 100x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 40 mL
  • Sample Diluent | 30 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 12 mL
  • Stop Solution | 12 mL
  • Plate Sealer | 3 pieces
Background CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2. CHK2 appears as a monomer in its inactive state. In the event of DNA damage the N-terminal SQ/TQ cluster domain (SCD) phosphorylation causes CHK2 dimerization. The phosphorylated Thr68 (located on the SCD) interacts with the central forkhead-associated (FHA) domain to form the dimer. After the protein dimerizes the kinase domain is activated via autophosphorylation. Once the kinase domain is activated the CHK2 dimer dissociates. Dimerization is a critical event in the activation of the Chk2 kinase. CHK2 is involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. Mutations to the CHEK2 gene have been linked to a wide range of cancers.
Gene Symbol CHEK2
Locus ID 11200
Standard Curve
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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