Human CD33 ELISA Kit 1 x 96

SKU EA200059

Human CD33 ELISA Kit 1 x 96

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 0.5 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 78-5000pg/ml
• Sample Type: Human serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of CD33
• Sensitivity: 22pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • CD33 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • Human CD33 Standard (250ng/ml) | 0.1 mL
  • 100x Biotin conjugated CD33 Detection Antibody | 0.12 mL
  • 100x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 40 mL
  • Sample Diluent | 30 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 12 mL
  • Stop Solution | 12 mL
  • Plate Sealer | 3 pieces
• Background: CD33 or Siglec-3 (sialic acid binding Ig-like lectin 3) is a transmembrane receptor expressed on cells of myeloid lineage. It binds sialic acids and is a member of the SIGLEC family of lectins. CD33 expression is restricted to early multilineage hematopoietic progenitors, myelomonocytic precursors and more mature myeloid cells, being absent on normal pluripotent hematopoietic stem cells. CD33 can be stimulated by any molecule with sialic acid residues such as glycoproteins or glycolipids, which results in a cascade that inhibits phagocytosis in the cell. About 85–90% of acute myeloid leukemia cases express the CD33 antigen. Circulating CD33 (cCD33) can be detected in the plasma from patients with leukemias, and cCD33 levels may have clinical implication such as predictive and prognostic value in these patients.
• Gene Symbol: CD33
• Locus ID: 945
• Shipping: Blue Ice