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Human AKT1 ELISA Kit 1 x 96

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SKU
EA200039
Human AKT1 ELISA Kit 1 x 96
  $570.00
4 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich
Assay Length 3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal Colorimetric
Curve Range 78-5000pg/ml
Sample Type Human serum, plasma, and other biological fluids.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of AKT1
Sensitivity 21pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • AKT1 Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • Human AKT1 Standard (250ng/ml) | 0.1 mL
  • 100x Biotin conjugated AKT1 Detection Antibody | 0.12 mL
  • 100x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 40 mL
  • Sample Diluent | 20 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 12 mL
  • Stop Solution | 12 mL
  • Plate Sealer | 3 pieces
Background AKT1 also known as RAC (Rho family)-alpha serine/threonine-protein kinase, is an enzyme that in humans is encoded by the AKT1 gene. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that belong to the AKT subfamily. AKT represents an important signaling hub, modulating many processes including metabolism, proliferation, cell survival, growth and angiogenesis by phosphorylating a range of downstream substrates in response to growth factor stimulation. In human cancers, AKT1 plays a central role in tumorigenesis. Recent studies using clinical specimens have shown that Akt protein is frequently activated in HER2/neu-overexpressing breast tumors. The overexpression of pAkt may be a powerful prognostic marker for predicting disease-free survival (DFS) and overall survival of breast cancer patients.
Gene Symbol AKT1
Locus ID 207
Standard Curve
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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