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Mouse Vegfa ELISA KIT (1 x 96 wells)

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SKU
EA102910
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Vegf/VEGFA with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Vegf/VEGFA tissue specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse VEGF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Specificity This kit is used for quantitative detection of Mouse VEGF
Sensitivity <2pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity|Volume
  • Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
  • Standard2
  • Antibody A|1|3ml
  • Antibody B|1|3ml
  • Sample Diluent|1|15ml
  • TBS-T Wash Buffer (25x)|1|12ml
  • Color Developing Reagent (TMB)|1|10ml
  • Stop Solution|1|10ml
  • Adhesive Plate Sealers|2|Piece
Background Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
Gene Symbol Vegfa
Locus ID 22339
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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