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Mouse Selp ELISA KIT (1 x 96 wells)

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SKU
EA102907
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse P Selectin/SELP with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma (citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. P Selectin/SELP tissue specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse P-Selectin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 312pg/ml-20000pg/ml
Specificity This kit is used for quantitative detection of Mouse P-Selectin
Sensitivity <5pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity|Volume
  • Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
  • Standard2
  • Antibody A|1|3ml
  • Antibody B|1|3ml
  • Sample Diluent|1|15ml
  • TBS-T Wash Buffer (25x)|1|12ml
  • Color Developing Reagent (TMB)|1|10ml
  • Stop Solution|1|10ml
  • Adhesive Plate Sealers|2|Piece
Background P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.
Gene Symbol Selp
Locus ID 20344
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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