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Mouse Sele ELISA KIT (1 x 96 wells)

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SKU
EA102903
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse E Selectin/SELE with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. E Selectin/SELE tissue specificity:
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse sE-Selectin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Specificity This kit is used for quantitative detection of Mouse sE-Selectin
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity
  • 96-well plate|1
  • lyophilized Standard
  • Biotinylated detection antibody (50x)|100 ul
  • Avidin-Biotin-Peroxidase Complex (30x)|400 µl
  • Sample diluent|30 ml
  • Antibody diluent|12 ml
  • Avidin-Biotin-Peroxidase Diluent|12 ml
  • Wash Buffer (25x)|20 ml
  • Color Developing Reagent (TMB)|10 ml
  • Stop Solution|10 ml
  • Plate Sealers|4
Background E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.
Gene Symbol Sele
Locus ID 20339
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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