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Human PDGFB ELISA KIT (1 x 96 wells)

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SKU
EA102901
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Pdgf Bb/PDGFB with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Pdgf Bb/PDGFB tissue specificity: Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. .
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human PDGF-AB
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Specificity This kit is used for quantitative detection of Human PDGF-AB
Sensitivity <2pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity
  • 96-well plate|1
  • lyophilized Standard
  • Biotinylated detection antibody (50x)|100 ul
  • Avidin-Biotin-Peroxidase Complex (30x)|400 µl
  • Sample diluent|30 ml
  • Antibody diluent|12 ml
  • Avidin-Biotin-Peroxidase Diluent|12 ml
  • Wash Buffer (25x)|20 ml
  • Color Developing Reagent (TMB)|10 ml
  • Stop Solution|10 ml
  • Plate Sealers|4
Background The platelet-derived growth factor(PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly(96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus(SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog(PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27KDa.
Gene Symbol PDGFB
Locus ID 5155, 5154
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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