Human GM CSF (CSF2) ELISA KIT (1 x 96 wells)

SKU
EA102892
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Gm-Csf/CSF2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Gm-Csf/CSF2 tissue specificity:
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human GM-CSF
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Specificity This kit is used for quantitative detection of Human GM-CSF
Sensitivity <1pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • Description|Quantity|Volume
  • Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells
  • Standard2
  • HRP-Linked Antibody|1|6ml
  • Sample Diluent|1|15ml
  • TBS-T Wash Buffer (25x)|1|12ml
  • Color Developing Reagent (TMB)|1|10ml
  • Stop Solution|1|10ml
  • Adhesive Plate Sealers|2|Piece
Background Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
Gene Symbol CSF2
Locus ID 1437
Shipping Blue Ice
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