Mouse Pla2g2a ELISA KIT (1 x 96 wells)

SKU
EA102631
For quantitative detection of mouse PLA2G2A in cell culture supernates, serum and plasma (heparin, EDTA).
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse PLA2G2A
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 31.2pg/ml-2000pg/ml
Specificity This kit is used for quantitative detection of Mouse PLA2G2A
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- mouse PLA2G2A antibody | 1
  • Lyophilized recombinant mouse PLA2G2A standard | 10ng/tube×2
  • Biotinylated anti- mouse PLA2G2A antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 4 D3; 4 70.57 cM. Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains.
Gene Symbol Pla2g2a
Locus ID 18780
Shipping Blue Ice
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