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Human PAM ELISA KIT (1 x 96 wells)

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SKU
EA102528
For quantitative detection of human PAM in cell culture supernates, serum and plasma (heparin, EDTA, citrate).
  $1,080.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human PAM
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 312pg/ml-20000pg/ml
Specificity This kit is used for quantitative detection of Human PAM
Sensitivity <50pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human PAM antibody | 1
  • Lyophilized recombinant human PAM standard | 20ng/tube×2
  • Biotinylated anti- human PAM antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Peptidyl-glycine alpha-amidating monooxygenase is an enzyme that catalyzes the biosynthesis of many signaling peptides and, in humans, is encoded by the PAM gene. It is mapped to 5q21.1. This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Gene Symbol PAM
Locus ID 5066
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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