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Mouse Pdgfb ELISA KIT (1 x 96 wells)

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SKU
EA102498
For quantitative detection of Mouse PDGF-BB in cell culture supernates, serum and plasma (heparin, EDTA).
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Mouse
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse PDGF-BB
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Specificity This kit is used for quantitative detection of Mouse PDGF-BB
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- mouse PDGF-BB antibody | 1
  • Lyophilized recombinant mouse PDGF-BB standard | 10ng/tubex2
  • Biotinylated anti- mouse PDGF-BB antibody | 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Gene Symbol Pdgfb
Locus ID 18591
Shipping Blue Ice
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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