Mouse Mpl ELISA KIT (1 x 96 wells)

SKU EA102312

For quantitative detection of mouse TPOR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Product Data

• Species: Mouse
• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for mouse TPOR
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 156pg/ml-10000pg/ml
• Specificity: This kit is used for quantitative detection of mouse TPOR
• Sensitivity: <15pg/ml
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- mouse TPOR antibody | 1
  • Lyophilized recombinant mouse TPOR standard | 10ng/tube×2
  • Biotinylated anti- mouse TPOR antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
• Background: The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
• Gene Symbol: Mpl
• Locus ID: 17480
• Shipping: Blue Ice