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Rat Col18a1 ELISA KIT (1 x 96 wells)

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SKU
EA102274
For quantitative detection of rat Endostatin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Rat
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for rat Endostatin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10000pg/ml
Specificity This kit is used for quantitative detection of rat Endostatin
Sensitivity <15pg/ml
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- rat Endostatin antibody | 1
  • Lyophilized recombinant rat Endostatin standard | 10ng/tube×2
  • Biotinylated anti- rat Endostatin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Gene Symbol Col18a1
Locus ID 85251
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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