Human dsDNA IgG ELISA kit

SKU EA100918

Human dsDNA IgG ELISA kit

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human dsDNA IgG
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with dsDNA antigen: 12x8x1
  • 2. Sample Diluent: 1 bottle (ready to use): 22 ml
  • 3. Enzyme conjugate: 1 bottle (ready to use): 12ml
  • 4. TMB Substrate: 1 bottle (ready to use): 12ml
  • 5. Calibrator: 1 Vial (ready to use): 1ml
  • 6. Positive Control: 1 vial (ready to use): 1ml
  • 7. Negative Control: 1 vial (ready to use: 1ml
  • 8. Stop Solution: 1 bottle (ready to use): 12ml
  • 9. Wash concentrate 20X: 1 bottle: 25ml
• Background: Anti-dsDNA is present in 50 % to 70% of patients with systemic lupus erythematosus (SLE). Circulating DNA/anti-DNA immune complexes are considered to play a part in the pathogenesis of SLE. The presence of anti-dsDNA is one of the diagnostic criteria for SLE. IgG antibodies to dsDNA are considered clinically most useful for the diagnosis and management of SLE. Antibodies to single stranded DNA (ssDNA) and IgM antibodies to DNA are found in a number of other connective diseases, liver diseases, as well as in some normal individuals. ELISA is the method of choice for the screening of anti-dsDNA in-patients with suspected SLE.
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