Human Creatine Kinase MB Isoenzyme (CKMB) ELISA kit

CAT#: EA100892

Human Creatine Kinase MB Isoenzyme (CKMB) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Specificity: This kit is used for quantitative detection human Creatine Kinase MB Isoenzyme (CKMB)
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Antibody-Coated Microtiter Plate: 12x8x1
  • 2. Liquid CK-MB Standards containing:0,7.5,15,50,100, & 200 ng/ml CK-MB. 1.0 ml for each standard dose. Store at -20?: 1ml
  • 3. Enzyme Conjugate Reagent, (Ready to Use): 22 ml
  • 4. TMB Reagent (one-step): 11ml
  • 5. Stop Solution: 11ml
  • 6. Wash Concentrate 20x: 1 Bottle: 25 ml
• Background: Creatine Kinase (CK-MB) is the enzyme being used as the definitive serum marker for the diagnosis or exclusion of acute myocardial infarction (AMI).The determination of CK-MB mass has proven to be more specific for myocardial necrosis than the long-standing CK-MB activity and CK-MB inhibition assays. CK- MB, released after AMI, is detectable in blood as early as 3-4 hours after the onset of symptoms, and remains elevated for approximately 65 hours post infarct 8-9. CK-MB mass levels are reportedly 50% diagnostic for AMI after 3 hours and > 90% diagnostic at 6 hours 10. Such accuracy makes CK-MB mass determinations useful in confirming AMI in patients presenting to the ER with non-diagnostic ECGs > 6 hours after the onset of symptoms.
• Gene Symbol: CKM
• Gene ID: 1158