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Human Androstenedione ELISA kit

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SKU
EA100852
Human Androstenedione ELISA kit
  $290.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 0.12-10 ng/mL
Sample Type Serum
Sample Volume 25 µl/well
Specificity This kit is used for quantitative detection human Androstenedione
Sensitivity 0.12 ng/mL
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 1. Microwells coated with Goat anti-rabbit IgG: 12x8x1
  • 2. Standard : 6 vials (ready to use): 0.5 ml
  • 3. Enzyme Conjugate (ready to use): 7 ml
  • 4. Rabbit Anti- Androstenedione Reagent (ready to use): 7 ml
  • 5. TMB substrate (ready to use): 12 ml
  • 6. Stop solution (ready to use): 12 ml
  • 7. Wash Solution 20x Concentrated: 25ml
Background Androstenedione is the primary precursor of testosterone in women. It is synthesized in the adrenal gland. Measurement of Androstenedione may be used as an indicator of androgenic activity in women. The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy. In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or maybe of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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