Human TGFBI/beta IG-H3 ELISA Kit

SKU
EA100707
Human TGFBI/beta IG-H3 ELISA Kit
  $755.00
3 Weeks*
Specifications
Specifications
Product Data
Species Human
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human TGFBI/betaIG-H3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human TGFBI
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human TGFBI antibody 1
  • Lyophilized recombinant human TGFBI standard 10ng/tubex2
  • Biotinylated anti- human TGFBI antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Transforming growth factor, beta-induced, 68kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Gene Symbol TGFBI
Locus ID 7045
Shipping Blue Ice
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Documents
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Full Issue PDF
JACC: Basic to Translational Science (2024) 9 (1), I-CLXV DOI: 10.1016/S2452-302X(23)00554-5
AI Summary: OriGene’s ELISA kits for TGFBI (EA100707) and FSTL3 (EA100552) were used in this study to quantitatively measure protein levels with high sensitivity.
Snippet: induced protein (TGFBI) (OriGene EA100707;detection limit: 10 pg/mL), follistatin-like 3 (FSTL3) (OriGene EA100552;detection limit: 10 pg/mL), stanniocalcin 2 (STC2) (Abcam ab222880;calculated minimal detectable dose: 0.95 ng/mL), and transg
Showing 1-1 of 1 papers.
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