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4C5, Anti-DDK Magnetic Immunoprecipitation Kit

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SKU
AR100024
4C5, Anti-DDK Magnetic Immunoprecipitation Kit
Size:
1 kit
  $688.00
2 Weeks*
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BRCA1-Associated Protein Increases Invasiveness of Esophageal Squamous Cell Carcinoma
Zhao, Wei, Shao et al
Gastroenterology (2017)
AI Summary: OriGene contributed to this study by providing Flag-tagged expression plasmids for PCKa (RC211893), PKCb (RC207217), PKCd (RC208127), PKCε (RC217702), PKCh (RC205272), PKCi (RC205379), and PKCz (RC202472), which were used to investigate the roles of these genes in esophageal squamous cell carcinoma invasiveness.
Snippet: The Flag-tagged expression plasmid for PCKa (RC211893), PKCb (RC207217), PKCd (RC208127), PKCε (RC217702), PKCh (RC205272), PKCi (RC205379), or PKCz (RC202472) was purchased from OriGene (Rockville, MD).
A Chaperone Complex Formed by HSP47, FKBP65 and BiP Modulates Telopeptide Lysyl Hydroxylation of Type I Procollagen
Duran, Martin, Weis et al
J. Bone Miner. Res. (2017)
AI Summary: OriGene products contributed to this study by providing the FKBP10-DDK-flag expression vector and the Immunoprecipitation and Detection Kit (AR100024), which were used for rescue experiments and protein detection, respectively.
Snippet: Rescue experiments were performed by electroporation of cells with a vector containing the FKBP10 coding sequence tagged with DDK-flag (Origene). ... Immunoprecipitation and detection kit (AR100024 Origene) were used. Control beads without anti-DDK or ... All 2 versions Cite SaveSaving
CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-β3 function
Yoshioka, Ono, Maeda et al
Bone (2016) 83, 162-70 DOI: 10.1016/j.bone.2015.11.007
Cited by 34
AI Summary: OriGene’s 4C5 anti-DDK mouse monoclonal antibody was used for immunoprecipitation and western blot analyses to detect DDK-tagged proteins, facilitating the study of CCN4’s interaction with TGF-β3 in chondrogenesis.
Snippet: Cleared cell lysates were incubated with magnetic beads conjugated with 4C5 anti-DDK mouse monoclonal antibody (OriGene, Rockville, MD, USA), or 1 μg mouse IgG1 kappa monoclonal [MOPC-21] isotype control (Abcam, Cambridge, UK) that was previously bound to Dynabeads protein G (Life Technologies). The beads were washed extensively, and the bound proteins were boiled in loading buffer for 10 min at 70 °C and then separated by SDS-PAGE and analyzed by immunoblot with 4C5 anti-DDK monoclonal antibody (OriGene) or anti-CCN4 antibody (LF-187). …
FigureFigure
IP WB
Germline Heterozygous Variants in SEC23B Are Associated with Cowden Syndrome and Enriched in Apparently Sporadic Thyroid Cancer
Yehia, Niazi, Ni et al
Am. J. Hum. Genet. (2015) 97 (5), 661-76 DOI: 10.1016/j.ajhg.2015.10.001
Cited by 92
AI Summary: OriGene’s anti-DDK (OriGene 4C5) antibody was used for protein pull-down and immunoblotting experiments to analyze SEC23B variants’ effects in thyroid cell lines, contributing to the study’s investigation of cancer-associated cellular mechanisms.
Snippet: … Protein lysates were collected by centrifugation at 13,000 RPM for 15 min at 4°C and pre-cleared by incubation with protein A/G PLUS-Agarose immunoprecipitation reagent (Santa Cruz sc-2003) for 30 min at 4°C on a rotator. Pre-cleared protein lysates were quantified with the BCA Protein Assay Kit (Thermo Scientific Pierce), and 1 mg/ml lysates were prepared. We used anti-SAR1A (Santa Cruz sc-130463) and anti-DDK (Origene 4C5) antibodies for pull-down and immunoblotting at the recommended dilutions
WB IP
Lysine Methyltransferase SETD7 (SET7/9) Regulates ROS Signaling through mitochondria and NFE2L2/ARE pathway
He, Owen, Jelinsky et al
Sci Rep (2015) 5, 14368 DOI: 10.1038/srep14368
Cited by 71
AI Summary: OriGene products, including DDK (FLAG)-tagged SETD7 human ORF clone, NFE2L2 human cDNA clone, pCMV6-DDK tagged empty vectors, and the Anti-DDK Magnetic Immunoprecipitation Kit, were used to express and purify proteins for studying the interaction between SETD7 and NFE2L2 in the regulation of ROS signaling.
Snippet: Plasmids and transfection. DDK (FLAG)-tagged SETD7 human ORF clone, NFE2L2 human cDNA clone and pCMV6-DDK tagged empty vectors were obtained from Origene. ... Protein pull-down was performed with Anti-DDK Magnetic Immunoprecipitation Kit (Origene ... Cite SaveSaving
FigureFigureFigure
IP
Gamma secretase-activating protein is a substrate for caspase-3: implications for Alzheimer's disease
Chu, Li, Joshi et al
Biol Psychiatry (2015) 77 (8), 720-8 DOI: 10.1016/j.biopsych.2014.06.003
Cited by 50
AI Summary: In this paper, OriGene’s anti-GSAP antibody (Cat# TA324482) was used to detect GSAP protein levels, enabling the authors to investigate caspase-3-dependent processing of GSAP and its role in amyloid-beta production relevant to Alzheimer’s disease.
FigureFigure
Showing 1-6 of 6 papers.
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