Streptavidin-HRP Conjugate

Snippet: ession levels were detected by IHC. The IHC sections were incubated overnight at 4°C in primary antibody solution containing anti-human TLR4 antibody (cat. no. A0456;final dilution, 2 µg/l;1:200;OriGene Technologies, Inc.), and a biotin-streptavidin HRP detection system for 12 h. The sections were incubated with biotinylated goat anti-rabbit IgG (cat. no. 2019629;1:200 dilution;OriGene Tech

Snippet: antigen retrieval was performed with citrate buffer for 15 min at 120°C. Endogenous peroxidase activity was blocked using 3% hydrogen peroxide in methanol for 10 min at room temperature. Goat serum (OriGene Technologies, Inc.) was used for blocking non-specific binding sites for 1–2 h at room temperature. Slides were then incubated with primary antibodies against HMGA2 (1:300 dilution;GTX10051

Snippet: … A TUNEL detection kit (OriGene Technologies, Inc.) was used to analyze the apoptosis of PC12 cells following LPS induction no. AR100017;OriGene Technologies, Inc.) and 3,3-diaminobenzidine color reagent (OriGene Technologies, Inc.);- TUNEL assay. … AR100017

Snippet: 96-well ELISA microplate was coated with anti-DDK antibody (Origene, TA50011-100) @ 1:200 dilution in coating buffer (0.1M carbonate, pH 9.6)