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CD3E Mouse Monoclonal Antibody [Clone ID: UMAB54]

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SKU
UM570048
CD3E mouse monoclonal antibody,clone UMAB54
Size:
30 ul 100 ul
Conjugation:
Unconjugated
  $224.00
4 Weeks*
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View other "CD3E or NM_000733" antibodies

Specifications
Specifications
Product Data
Clone Name UMAB54
Application IF, Simple Western, WB
Recommended Dilution WB 1:500, IF 1:100 ; Simple Western 1:20-1:50
Reactivity Human
Antibody Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
Buffer PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration 0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Predicted Protein Size 20.7 kDa
Gene Name CD3e molecule
Database Link
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. provided by RefSeq, Jul 2008
Synonyms IMD18; T3E; TCRE
Reference Data
Protein Categories ADC Targets, CD Markers, Immune system diseases, Membrane Proteins
Protein Families Druggable Genome, Transmembrane
Protein Pathways Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway
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Other "UMAB54" antibodies (2)
SKU Description Size Price
UM500048 CD3E mouse monoclonal antibody,clone UMAB54 100 ul
$610.00
UM500048CF Carrier-free (BSA/glycerol-free) CD3E mouse monoclonal antibody,clone UMAB54 100 ug
$680.00

file-alt Citations

Multiomic analysis identifies suppressive myeloid cell populations in human TB granulomas
Jain, Ogbonna, Maliga et al
bioRxiv (2025)
Cited by 1
AI Summary: OriGene's anti-CD3E antibody (Clone UMAB54, UM500048) was used for immunostaining to identify T cells within human TB granuloma tissue, facilitating the spatial transcriptional and immunophenotyping analyses described in the study.
Snippet: sc-20060AF594)/CD163(clone EPR14643-36, 6 Abcam ab272103), AF647(red):CD3E(Clone UMAB54, Origene, UM500048), AF488(green):Vimentin(Clone 7 E5, Santa Cruz, sc-373717), in combination with H&E staining and pathologist opinion to select the Region of
IHC FC
HIV persists in late coronary atheroma and is associated with increased local inflammation and disease progression
Wanjalla, Obare, Bailin et al
Res Sq (2024)
Cited by 2
Snippet: They were stained with fluorescent anti-CD3 632 (Origene, Cat# UM500048, clone UMAB54), anti-CD8 (Invitrogen, Cat# 14-0008-82, clone 633 AMC908), anti- CD68 (Satna Cruz, Cat.# sc-20060, clone KP1) and SYTO 83 nuclear staining 634 (Invitrogen, Cat# S11364). Fluorescence microscopy on the GeoMX platform was used to image
FigureFigureFigure
IF
Preexisting Skin-Resident CD8 and γδ T-cell Circuits Mediate Immune Response in Merkel Cell Carcinoma and Predict Immunotherapy Efficacy
Reinstein, Zhang, Ospina et al
Cancer Discov (2024) 14 (9), 1631-1652 DOI: 10.1158/2159-8290.CD-23-0798
AI Summary: OriGene’s CD3 antibody (Clone: UMAB54; ID: UM500048) was used to identify and analyze T cells within tumor samples, enabling the study of immune cell circuits that predict immunotherapy response in Merkel cell carcinoma.
Snippet: The markers used in the ROI selection were SYTO13 (DNA/nuclei), KRT18 (keratin/cancer cells), PTPRC (CD45/lymphocytes), and CD3E (T cells). …Protein: CD3;Manufacturer: Origene;Clone: UMAB54;ID: UM500048, RRID:AB_2629062
Spatially resolved analysis of pancreatic cancer identifies therapy-associated remodeling of the tumor microenvironment
Shiau, Cao, Gong et al
Nat Genet (2024)
Cited by 92
AI Summary: OriGene’s anti-CD3 antibody (cat. no. UMAB54) was used for immunofluorescent labeling to identify CD3-positive cells during spatial analysis of the pancreatic tumor microenvironment in this study.
Snippet: orophore-conjugated antibody cocktail against CD298 (Abcam, cat. no. EP1845Y), B2M (Abcam, cat. no. EP2978Y), pan-cytokeratin (PanCK;Novus, cat. no. AE-1/AE-3), CD45 (Novus, cat no. 2B11+PD7/26) and CD3 (Origene, cat. no. UMAB54) proteins and DAPI. Image processing and cell segmentation Raw image processing and feature extraction were performed using an in-house SMI data processing pipeline19 whi...
Safety and reactogenicity of a controlled human infection model of sand fly-transmitted cutaneous leishmaniasis
Parkash, Ashwin, Dey et al
Nat Med (2024)
Cited by 25
AI Summary: OriGene's mouse anti-human CD3 antibody (UM500048CF) was used in this study to stain tissue sections for immunohistological analysis of immune cell distribution in human cutaneous leishmaniasis lesions.
Snippet: Sections were stained with the following primary antibodies overnight at 4 °C: mouse anti-human CD3 (1:100, OriGene, UM500048CF);rabbit anti-CD4 (1:50, Abcam, ab133616);mouse anti-CD8 (1:100, BioLegend, 372902);rabbit anti-human CD68 (1:800, Abcam, ab213363)
IHC
Characterisation of HBV and co-infection with HDV and HIV through spatial transcriptomics
Cross, Harris, Arbe-Barnes et al
egastro (2024) 2 (3), e100067 DOI: 10.1136/egastro-2024-100067
Cited by 15
Snippet: … MAN-10 131-02). The panel of morphology markers was designed to include HBsAg ((Polyclonal) 488 1:200 from Bioss #bs-1557G-A488), CD45 (D9M8I) 594 1:100 from Cell Signaling Technology #13 917BF, CD3 (clone UMAB54;Origene #UM500048CF) and a DNA dye (Syto83dye;Invitrogen). …
IHC
Showing 1-6 of 24 papers.
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