PSMA (FOLH1) Mouse Monoclonal Antibody [Clone ID: UMAB26]

CAT#: UM570026

FOLH1 mouse monoclonal antibody,clone UMAB26

Product Images

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB26, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB26, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB26, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-FOLH1 (PSMA) clone UMAB26 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500026 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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  Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-FOLH1 (PSMA) clone UMAB26 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500026 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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  Western blot of cell lysates (35ug) from MDCK. Diluation: 1:500

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

• Western blot of mouse tissue lysates (20ug) from Brain. Diluation: 1:500.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOLH1 mouse monoclonal antibody (Clone UMAB26). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-FOLH1 (Clone UMAB26) very specifically recognizes FOLH1 antigen on OriGene protein microarray chip.

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Specifications

Product Data

• Clone Name: UMAB26
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.2 kDa
• Gene Name: Homo sapiens folate hydrolase 1 (FOLH1), transcript variant 1, mRNA.
• Database Link:
  NP_004467
  Entrez Gene 2346 Human
  UniProt ID Q04609
• Background: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq]
• Synonyms: FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane