SARS-CoV-2 Spike Protein Rabbit Polyclonal Antibody

Snippet: No. AF933;1:1000 for Western blotting) was purchased from R&D Systems.Rabbit polyclonal anti–SARS-CoV-2 spike protein (Cat. No. TA890227;1:2000 for Western blotting) was from OriGene.SARS-CoV-2 spike guinea pig antibody (Cat. No. NR-10361;1:15,000 for plaque assay) was obtained from BEI Resources.Horseradish peroxidase (HRP)–conjugated go

Snippet: pseudovirus particles in brain organoids Brain organoids were fixed with 4% polyformaldehyde, washed with PBS, and blocked with 1% BSA. Then, organoids were incubated with an anti-S antibody (1:2000;OriGene, TA890227), washed with PBS, and treated with a gold-labeled secondary antibody. Subsequent steps included another round of fixation, washing, and staining with uranyl acetate. The organoids u

Snippet: … Cells were incubated with rabbit polyclonal antibodies against spike glycoprotein and ACE2 (Cat # TA890227, TA306149, OriGene Technologies, Rockville, MD, USA) diluted in blocking buffer for 2 h at room temperature. Plates were washed 3× with wash buffer (0.05% tween 20 in tris buffered saline) and incubated with;TA306149, OriGene Technologies, Rockville, MD, USA) diluted in blocking buffer for 2 h at room temperature. Plates were washed 3× with wash buffer (0.05% tween 20 in tris buffered saline) and incubated with 0.1 μg/ml peroxidase-conjugated goat anti-rabbit IgG (Cat #TA140003, OriGene Technologies