ABCD1 Mouse Monoclonal Antibody [Clone ID: OTI4C2]

Snippet: We overexpressed these UAS constructs in a similar fashion like the Abcd1 - knockdown. Supp. Fig. 7 shows western blot of human ABCD1, using an anti -ABCD1 (Origene #TA803208) antibody, verifying its expression in the flies. Taken together, these data suggest that overexpression of the

Snippet: each), and blocking agent was applied. Incubation was followed at room temperature for 45 minutes in 5% BSA in PBS with 3% normal goat serum (Vector Lab, #S-1000). Primary antibodies (Mouse ABCD1, Origene, TA803208, 1:250, Rabbit (alternating): IBA1, Wako, 019-19741, 1:200 and Von Willebrand Factor, Abcam, ab6994, 1:500) were applied in 3% BSA solution in PBS, and incubated o/n at 4 degrees. Pr

Snippet: Leica 1860, Leica biosystem, MI, Italy). The immunohistochemistry staining was performed with 484 mouse anti-human ABCD1 antibody (1:500, Origene, Rockville, MD), as well as secondary 485 antibodies such as Alexa Fluor 594 goat anti-mouse (1:400, Invitrogen;The immunohistochemistry staining was performed with mouse anti-human ABCD1 antibody (1:500, Origene, Rockville, MD),

Snippet: … Membranes were blocked in TBS-T (1% Tween 20) with 5% BSA for 30 min and incubated with primary antibody against human ABCD1 (1:1,000;Origene, MD, US;Cat. … TA803208) and;- by electrophoresis and transferred to polyvinylidene fluoride membranes. Membranes were blocked in TBS-T (1% Tween 20) with 5% BSA for 30 min and incubated with primary antibody against human ABCD1 (1:1,000

Snippet: Membranes were blocked with 5% non‑fat milk in phosphate‑buffered saline containing 0.05% Tween‑20 at room temperature for 2 h and probed with antibodies at 4˚C for 14 h including Anti‑ABCD1 (1:1,000;OriGene Technologies, Inc.;cat. no