CD8A Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3H6]

Snippet: CD8 antibody used CF802079 from Origene. Images were processed in QuPath, where cells were segmented using the built-in cell detection algorithm and PVR, TIGIT, or

Snippet: pression. Antibodies Syto13-FITC (nucleic acid stain) and PanCK-Cy3 were part of the Nanostring GeoMx kit. An anti-human CD8a (Clone ID: OTI3H6) monoclonal antibody (Cat# CF802079) was purchased from Origene and conjugated with AF647 using Alexa Fluor 647 antibody labeling kit (Cat# A20186, Thermo Fisher Scientific) following the manufacturer’s protocol. Nanostring GeoMx proteomics analysis Slid

Snippet: perature and then washed three times with 1X PBS. To block non-specific binding sites, the slides were incubated in 5% BSA in PBS for 1 h at room temperature. Primary antibodies, anti-CD8 (CF802079;OriGene, dilution 1:100, [OTI3H6]) and anti-pSTAT1 (9167 S;Cell Signaling TECHNOLOGY, dilution 1:50, [58D6]) were diluted in 2% BSA in PBS. Subsequently the slides were incubated in a humid chamber

Snippet: s (CD8), NK cells (CD56), macrophages (CD68) and plasma (CD138). These TIICs were stained with different antibodies as follows: an anti-human CD8 mouse monoclonal antibody (TA802079S, dilution 1:150, Origene), an anti-human CD56 mouse monoclonal antibody (TA805376S, dilution 1:150, Origene), an anti-human CD68 mouse monoclonal antibody (TA802949S, dilution 1:500, Origene), and an anti-human CD138;- Origene), an anti-human CD56 mouse monoclonal antibody (TA805376S, dilution 1:150, Origene (TA802949S, dilution 1:500, Origene), and an anti-human CD138 mouse Save Cached

Snippet: … Primary antibodies and dilutions used were as follows: ATF3 (Novus Biologicals, NBP1-85816, [1:50]) AREG (Proteintech, 16036-1-AP, [1:50]) CD8 (Origene, TA802079, [1:50]) Microscopy Images were obtained from a Zeiss Axio Imager M1 and were acquired using diode lasers 402, 488, and 561. To obtain images for CD8+ cell counting, five randomly s;Primary antibodies and dilutions used were as follows: ATF3 (Novus Biologicals, NBP1-85816, [1:50]) AREG (Proteintech, 16036-1-AP, [1:50]) CD8 (Origene, TA802079, [1:50]) Microscopy