Tapasin Related Protein (TAPBPL) Mouse Monoclonal Antibody [Clone ID: OTI4H5]

CAT#: TA505566

TAPBPL mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TAPBPL (Cat# RC201899, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAPBPL(Cat# TA505566). Positive lysates LY413343 (100ug) and LC413343 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TAPBPL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TAPBPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505566)

• Anti-TAPBPL mouse monoclonal antibody (TA505566) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TAPBPL (RC201899).

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Specifications

Product Data

• Clone Name: OTI4H5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TAPBPL(NP_060479) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: TAP binding protein like
• Database Link:
  NP_060479
  Entrez Gene 712626 MonkeyEntrez Gene 55080 Human
  UniProt ID Q9BX59
• Background: Tapasin, or TAPBP (MIM 601962), is a member of the variable-constant Ig superfamily that links major histocompatibility complex (MHC) class I molecules to the transporter associated with antigen processing (TAP; see MIM 170260) in the endoplasmic reticulum (ER). The TAPBP gene is located near the MHC complex on chromosome 6p21.3. TAPBPL is a member of the Ig superfamily that is localized on chromosome 12p13.3, a region somewhat paralogous to the MHC. [supplied by OMIM, Mar 2008]
• Synonyms: TAPBP-R; TAPBPR

Reference Data

• Protein Families: Druggable Genome, Transmembrane