AKT1 Mouse Monoclonal Antibody [Clone ID: OTI4D6]
$200.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI4D6 |
|---|---|
| Application | FC, IF, IHC, Simple Western, WB |
| Recommended Dilution | WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100 ; Simple Western 1:20-1:50 |
| Reactivity | Dog, Human, Monkey, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human AKT1(NP_005154) produced in HEK293T cell. |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | AKT serine/threonine kinase 1 |
| Database Link | |
| Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. provided by RefSeq |
| Synonyms | AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
| Note | This antibody also cross-reacts with AKT3. |
| Reference Data | |
| Protein Categories | Cancer: Brain and CNS, Cancer: Gynecologic, Cytokines, Enzyme: Kinases, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI4D6" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF504230 | Carrier-free (BSA/glycerol-free) AKT1 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6) | 100 ug |
$620.00
|
|
| TA504230 | AKT1 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6) | 100 ul |
$447.00
|
|
| TA504230AM | AKT1 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6), Biotinylated | 100 ul |
$509.00
|
|
| TA504230BM | AKT1 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6), HRP conjugated | 100 ul |
$509.00
|
|
Citations
Sitravatinib combined with osimertinib overcomes acquired osimertinib resistance in NSCLC by targeting AXL and the PI3K/AKT/GSK3β signaling pathway
Wang, Chen, Wang et al
Scientific Reports (2026)
Wang, Chen, Wang et al
Scientific Reports (2026)
AI Summary: In this study, OriGene products—specifically the Snail antibody (TA500366S) and AKT antibody (TA504230S)—were used in Western blot analyses to investigate molecular mechanisms underlying the reversal of osimertinib resistance in NSCLC.
Snippet: vernight with the following designated primary antibodies on a shaker: AXL (Affbiotech, #AF7793, China), phospho-AXL (p-AXL;Affbiotech, #AF8523, China), Vimentin (Abcam, #92547, USA), E-cadherin (HuaBIO, #M1405-3, China), N-cadherin (HuaBIO, #ET1607-37, China), Snail (Origene, #TA500366S, China), AKT (Origene, #TA504230S, China), phospho-AKT (p-AKT;Promab
GAS reduced inflammatory responses in activated microglia by regulating the Ccr2/Akt/Gsk-3β pathway
Shi, Shi, Wang et al
Mol Brain (2025) 18 (1), 40 DOI: 10.1186/s13041-025-01206-w
Shi, Shi, Wang et al
Mol Brain (2025) 18 (1), 40 DOI: 10.1186/s13041-025-01206-w
Cited by 1
AI Summary: OriGene products, including antibodies against Ccr2 (TA350635), Akt (TA504230), p-Akt (TA325218), and Gsk3 beta (TA367586), were used to detect and quantify key proteins involved in the Ccr2/Akt/Gsk-3β pathway, enabling the study to elucidate how Gastrodin (GAS) modulates inflammatory responses in activated microglia.
Snippet: were transferred onto polyvinylidene fluoride (PVDF) membranes and blocked with skim milk. They were incubated overnight at 4 °C with the following antibodies: anti-Ccr2 (1:2000;Rabbit polyclonal;ORIGENE;TA350635), Akt (1:2000;Mouse monoclonal;TA504230), p-Akt (1:2000;TA325218), anti-Gsk3 beta (1:1000;TA367586), anti-phospho
Gastrodin Regulates PI3K/AKT-Sirt3 Signaling Pathway and Proinflammatory Mediators in Activated Microglia
Zuo, Wang, Ren et al
Mol Neurobiol (2023)
Zuo, Wang, Ren et al
Mol Neurobiol (2023)
Cited by 24
AI Summary: OriGene products—anti-PI3K (AP20772PU-N), anti-phosphorylated PI3K (TA325781), AKT (TA504230), and phosphorylated AKT (TA325218) antibodies—were used in this study to detect and analyze key proteins involved in the PI3K/AKT-Sirt3 signaling pathway, helping elucidate gastrodin's neuroprotective mechanisms in activated microglia.
Snippet: Santa Cruz Biotechnology, Santa Cruz, CA, USA;sc-32,725), anti-PI3K (1:200;Rabbit polyclonal;Origene Technologies, Rockville, MD, USA;AP20772PU-N), anti-phosphorylated (P)-PI3K (1:200;ORIGENE;TA325781), AKT (1:200;Mouse monoclonal;Origene Technologies;TA504230) and P-AKT (1:200;TA325218). After washing with PBS, the slides were
Evidence for a PI3-kinase independent pathway in the regulation of Rap1b activation downstream of the P2Y12 receptor in platelets
Dangelmaier, Kunapuli
Platelets (2022)
Dangelmaier, Kunapuli
Platelets (2022)
Cited by 6
AI Summary: OriGene's total Akt antibody (cat# TA504230) was used in this study to detect Akt protein levels, enabling the assessment of PI3-kinase pathway activation in platelet signaling experiments.
Snippet: Antibody to total Akt cat#TA504230 was purchased from Origene (city state). Blocking buffer, infrared dye-labeled goat anti-mouse (926-68020) and anti-rabbit antibodies (
miR-335-laden B Cell-Derived Extracellular Vesicles Promote SOX4-Dependent Apoptosis in Human Multiple Myeloma Cells
Lombardi, Almanza, Kowal et al
J Pers Med (2021) 11 (12) DOI: 10.3390/jpm11121240
Lombardi, Almanza, Kowal et al
J Pers Med (2021) 11 (12) DOI: 10.3390/jpm11121240
Cited by 10
AI Summary: In this study, OriGene’s SOX4 (Cat# RC218364) and HIF1A (Cat# RC221345) expression plasmids were used to investigate the regulatory effects of miR-335 on SOX4 and HIF1A in multiple myeloma cell models.
Protease-activated receptor 4 causes Akt phosphorylation independently of PI3 kinase pathways
Dangelmaier, Kunapuli
Platelets (2020)
Dangelmaier, Kunapuli
Platelets (2020)
Cited by 6
AI Summary: In this study, the total Akt antibody (cat# TA504230) from OriGene was used to detect Akt protein levels, enabling the researchers to assess Akt phosphorylation in platelet signaling pathways.
Showing 1-6 of 8 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
