GSPT2 Mouse Monoclonal Antibody [Clone ID: OTI2G9]

CAT#: TA503066

GSPT2 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSPT2 (Cat# RC206259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSPT2(Cat# TA503066). Positive lysates LY402649 (100ug) and LC402649 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GSPT2 monoclonal antibody (1:200).

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  Anti-GSPT2 mouse monoclonal antibody (TA503066) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSPT2 (RC206259).

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  Immunofluorescent staining of HeLa cells using anti-GSPT2 mouse monoclonal antibody (TA503066).

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  HEK293T cells transfected with either RC206259 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSPT2 antibody (TA503066), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-GSPT2 antibody (TA503066), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2G9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GSPT2 (NP_060564) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.32 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.7 kDa
• Gene Name: G1 to S phase transition 2
• Database Link:
  NP_060564
  Entrez Gene 14853 MouseEntrez Gene 501582 RatEntrez Gene 23708 Human
  UniProt ID Q8IYD1
• Background: This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability. COMPLETENESS: complete on the 3' end.
• Synonyms: ERF3B; GST2