Chk2(CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CAT#: TA500398

CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)

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 Product Datasheet for 'TA500398'

USD 379.00


Availability*
In Stock

Size
    • 100 ul

Specifications

Product Data
Clone Name OTI5C4
Applications WB, IHC, IF, IP, FC
Recommend Dilution WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Reactivity Human , Dog
Host Mouse
Clonality Monoclonal
Immunogen Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Isotype IgG1
Formulation PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Reconstitution Method Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Concentration 1.43 mg/ml
Purification Purified from mouse ascites fluids by affinity chromatography
Predicted Protein Size 60.7 kDa
Gene Name checkpoint kinase 2
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Synonyms CDS1|CHK2|HuCds1|LFS2|PP1425|RAD53|hCds1
Reference Data
Protein Families Protein Kinase, Stem cell - Pluripotency, Druggable Genome
Protein Pathways Cell cycle, p53 signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.