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alpha Actinin (ACTN1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7A4]

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SKU
TA500072BM
alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4), HRP conjugated
Size:
100 ul
Conjugation:
HRP Unconjugated Biotin
  $509.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

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View other "alpha Actinin or NM_001102" antibodies

Specifications
Specifications
Product Data
Clone Name OTI7A4
Application IF, Simple Western, WB
Recommended Dilution WB 1:2000, IF 1:100, Simple Western 1:20 - 1:50
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.
Buffer PBS (pH 7.3) containing 1% BSA and 50% glycerol
Concentration 0.5 mg/ml
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation HRP
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Predicted Protein Size 102.9 kDa
Gene Name actinin alpha 1
Database Link
Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Synonyms BDPLT15
Reference Data
Protein Categories Cardiovascular diseases, Intracellular Proteins
Protein Families Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
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Other "OTI7A4" antibodies (4)
SKU Description Size Price
CF500072 Carrier-free (BSA/glycerol-free) alpha-actinin mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) 100 ug
$620.00
TA500072 alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) 100 ul
$447.00
TA500072AM alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4), Biotinylated 100 ul
$509.00
TA500072S alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) 30 ul
$200.00

file-alt Citations

Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells
Xinaris, Benedetti, Novelli et al
J. Am. Soc. Nephrol. (2016) 27 (5), 1400-11 DOI: 10.1681/ASN.2015030316
AI Summary: OriGene’s rabbit anti–α-actinin-4 antibody was used in this study to identify and characterize podocyte differentiation within kidney organoids generated from amniotic fluid stem cells.
Snippet: rabbit anti–α-actinin-4 (1:50;OriGene Technologies, Inc., Rockville, MD), rabbit anti-human nestin (1:300, Merck Millipore),
Involvement of Ubiquitin-Conjugating Enzyme E2C in Proliferation and Invasion of Prostate Carcinoma Cells
Shuliang, Lei, Guangwu et al
Oncol. Res. (2014) 21 (3), 121-7 DOI: 10.3727/096504013X13832473329953
AI Summary: OriGene’s anti-actin antibody was used in this study to detect actin as a loading control in Western blot analyses, helping to assess protein expression changes related to UBE2C’s role in prostate cancer cell proliferation and invasion.
Snippet: Then the membrane was probed overnight at 4°C in blocking buffer with primary antibodies: actin (1:2,000, Origene), E-cadherin (1:1,000, Cell Signaling Technology), vimentin (1:1,000, Cell Signaling Technology), fibronectin (1:5,000, Cell Signaling Technology), UBE2C ... Cite SaveSaving
WB
Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Surcel, Schiffhauer, Thomas et al
bioRxiv (2017)
Snippet: Antibodies used include: myosin IIA Poly19098 (BioLegend, 909801), myosin IIB (D8H8) XP (Cell Signaling Technology, #8824), myosin IIC (D4A7) (Cell Signaling Technology, #8189), α- actinin 1 (OTI7A4) (OriGene, TA500072), α-actinin 4 (G-4) (Santa Cruz Biotechnology ...;... Antibodies used include: myosin IIA Poly19098 (BioLegend, 909801), myosin IIB (D8H8) XP (Cell Signaling Technology, #8824), myosin IIC (D4A7) (Cell Signaling Technology, #8189), α- actinin 1 (OTI7A4) (OriGene, TA500072), α-actinin 4 (G-4) (Santa Cruz Biotechnology ...;... Antibodies used include: myosin IIA Poly19098 (BioLegend, 909801), myosin IIB (D8H8) XP (Cell Signaling Technology, #8824), myosin IIC (D4A7) (Cell Signaling Technology, #8189), α- actinin 1 (OTI7A4) (OriGene, TA500072), α-actinin 4 (G-4) (Santa Cruz Biotechnology
Showing 1-3 of 3 papers.
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